Shotgun Quantitative Proteomic Analysis of Proteins Responding to Drought Stress in Brassica rapa L. (Inbred Line “Chiifu”)
Joint Authors
Kim, Mijeong
Kim, Hijin
Kwon, Soon-Wook
Lee, Joohyun
Source
International Journal of Genomics
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Through a comparative shotgun quantitative proteomics analysis in Brassica rapa (inbred line Chiifu), total of 3,009 nonredundant proteins were identified with a false discovery rate of 0.01 in 3-week-old plants subjected to dehydration treatment for 0, 24, and 48 h, plants subjected to drought stress.
Ribulose-bisphosphate carboxylases, chlorophyll a/b-binding protein, and light harvesting complex in photosystem II were highly abundant proteins in the leaves and accounted for 9%, 2%, and 4%, respectively, of the total identified proteins.
Comparative analysis of the treatments enabled detection of 440 differentially expressed proteins during dehydration.
The results of clustering analysis, gene ontology (GO) enrichment analysis, and analysis of composite expression profiles of functional categories for the differentially expressed proteins indicated that drought stress reduced the levels of proteins associated with photosynthesis and increased the levels of proteins involved in catabolic processes and stress responses.
We observed enhanced expression of many proteins involved in osmotic stress responses and proteins with antioxidant activities.
Based on previously reported molecular functions, we propose that the following five differentially expressed proteins could provide target genes for engineering drought resistance in plants: annexin, phospholipase D delta, sDNA-binding transcriptional regulator, auxin-responsive GH3 family protein, and TRAF-like family protein.
American Psychological Association (APA)
Kwon, Soon-Wook& Kim, Mijeong& Kim, Hijin& Lee, Joohyun. 2016. Shotgun Quantitative Proteomic Analysis of Proteins Responding to Drought Stress in Brassica rapa L. (Inbred Line “Chiifu”). International Journal of Genomics،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1106158
Modern Language Association (MLA)
Kwon, Soon-Wook…[et al.]. Shotgun Quantitative Proteomic Analysis of Proteins Responding to Drought Stress in Brassica rapa L. (Inbred Line “Chiifu”). International Journal of Genomics No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1106158
American Medical Association (AMA)
Kwon, Soon-Wook& Kim, Mijeong& Kim, Hijin& Lee, Joohyun. Shotgun Quantitative Proteomic Analysis of Proteins Responding to Drought Stress in Brassica rapa L. (Inbred Line “Chiifu”). International Journal of Genomics. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1106158
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106158