De Novo Sequencing and Characterization of the Transcriptome of Dwarf Polish Wheat (Triticum polonicum L.)
Joint Authors
Wang, Xiaolu
Wang, Yi
Kang, Houyang
Fan, Xing
Sha, Lina
Zhang, Haiqin
Zhou, Yonghong
Xiao, Xue
Zeng, Jian
Wang, Chao
Peng, Fan
Wang, Ruijiao
Jiang, Yulin
Source
International Journal of Genomics
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-27
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Construction as well as characterization of a polish wheat transcriptome is a crucial step to study useful traits of polish wheat.
In this study, a transcriptome, including 76,014 unigenes, was assembled from dwarf polish wheat (DPW) roots, stems, and leaves using the software of Trinity.
Among these unigenes, 61,748 (81.23%) unigenes were functionally annotated in public databases and classified into differentially functional types.
Aligning this transcriptome against draft wheat genome released by the International Wheat Genome Sequencing Consortium (IWGSC), 57,331 (75.42%) unigenes, including 26,122 AB-specific and 2,622 D-specific unigenes, were mapped on A, B, and/or D genomes.
Compared with the transcriptome of T.
turgidum, 56,343 unigenes were matched with 103,327 unigenes of T.
turgidum.
Compared with the genomes of rice and barley, 14,404 and 7,007 unigenes were matched with 14,608 genes of barley and 7,708 genes of rice, respectively.
On the other hand, 2,148, 1,611, and 2,707 unigenes were expressed specifically in roots, stems, and leaves, respectively.
Finally, 5,531 SSR sequences were observed from 4,531 unigenes, and 518 primer pairs were designed.
American Psychological Association (APA)
Wang, Yi& Wang, Chao& Wang, Xiaolu& Peng, Fan& Wang, Ruijiao& Jiang, Yulin…[et al.]. 2016. De Novo Sequencing and Characterization of the Transcriptome of Dwarf Polish Wheat (Triticum polonicum L.). International Journal of Genomics،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1106169
Modern Language Association (MLA)
Wang, Yi…[et al.]. De Novo Sequencing and Characterization of the Transcriptome of Dwarf Polish Wheat (Triticum polonicum L.). International Journal of Genomics No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1106169
American Medical Association (AMA)
Wang, Yi& Wang, Chao& Wang, Xiaolu& Peng, Fan& Wang, Ruijiao& Jiang, Yulin…[et al.]. De Novo Sequencing and Characterization of the Transcriptome of Dwarf Polish Wheat (Triticum polonicum L.). International Journal of Genomics. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1106169
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106169