Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense
Joint Authors
Qin, Jian G.
Kong, Youqin
Chen, Liqiao
Ding, Zhili
Sun, Shengming
Wang, Ligai
Ye, Jinyun
Source
International Journal of Genomics
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-13
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Hemocyanin is a copper-containing protein with immune function against disease.
In this study, a hemocyanin subunit named MnHc-1 was cloned from Macrobrachium nipponense.
The full-length cDNA of MnHc-1 was 2,163 bp with a 2,028-bp open reading frame (ORF) encoding a polypeptide of 675 amino acids.
The MnHc-1 mRNA was expressed in the hepatopancreas, gill, hemocytes, intestine, ovary, and stomach, with the highest level in the hepatopancreas.
In the infection trial, the MnHc-1 mRNA transcripts in the hemocytes were significantly downregulated at 3 h after injection of Aeromonas hydrophila and then upregulated at 6 h and 12 h, followed by a gradual recovery from 24 to 48 h.
The MnHc-1 transcriptional expression in the hepatopancreas was measured after M.
nipponense were fed seven diets with 2.8, 12.2, 20.9, 29.8, 43.1, 78.9, and 157.1 mg Cu kg−1 for 8 weeks, respectively.
The level of MnHc-1 mRNA was significantly higher in the prawns fed 43.1–157.1 mg Cu kg−1 diet than in that fed 2.8–29.8 mg Cu kg−1 diet.
This study indicated that the MnHc-1 expression can be affected by dietary copper and the hemocyanin may potentially participate in the antibacterial defense of M.
nipponense.
American Psychological Association (APA)
Kong, Youqin& Chen, Liqiao& Ding, Zhili& Qin, Jian G.& Sun, Shengming& Wang, Ligai…[et al.]. 2016. Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense. International Journal of Genomics،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1106173
Modern Language Association (MLA)
Kong, Youqin…[et al.]. Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense. International Journal of Genomics No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1106173
American Medical Association (AMA)
Kong, Youqin& Chen, Liqiao& Ding, Zhili& Qin, Jian G.& Sun, Shengming& Wang, Ligai…[et al.]. Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense. International Journal of Genomics. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1106173
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106173