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Effect of Trehalose and Trehalose Transport on the Tolerance of Clostridium perfringens to Environmental Stress in a Wild Type Strain and Its Fluoroquinolone-Resistant Mutant
Joint Authors
Park, Miseon
Rafii, Fatemeh
Mitchell, Wilfrid J.
Source
International Journal of Microbiology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Trehalose has been shown to protect bacterial cells from environmental stress.
Its uptake and osmoprotective effect in Clostridium perfringens were investigated by comparing wild type C.
perfringens ATCC 13124 with a fluoroquinolone- (gatifloxacin-) resistant mutant.
In a chemically defined medium, trehalose and sucrose supported the growth of the wild type but not that of the mutant.
Microarray data and qRT-PCR showed that putative genes for the phosphorylation and transport of sucrose and trehalose (via phosphoenolpyruvate-dependent phosphotransferase systems, PTS) and some regulatory genes were downregulated in the mutant.
The wild type had greater tolerance than the mutant to salts and low pH; trehalose and sucrose further enhanced the osmotolerance of the wild type to NaCl.
Expression of the trehalose-specific PTS was lower in the fluoroquinolone-resistant mutant.
Protection of C.
perfringens from environmental stress could therefore be correlated with the ability to take up trehalose.
American Psychological Association (APA)
Park, Miseon& Mitchell, Wilfrid J.& Rafii, Fatemeh. 2016. Effect of Trehalose and Trehalose Transport on the Tolerance of Clostridium perfringens to Environmental Stress in a Wild Type Strain and Its Fluoroquinolone-Resistant Mutant. International Journal of Microbiology،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1106270
Modern Language Association (MLA)
Park, Miseon…[et al.]. Effect of Trehalose and Trehalose Transport on the Tolerance of Clostridium perfringens to Environmental Stress in a Wild Type Strain and Its Fluoroquinolone-Resistant Mutant. International Journal of Microbiology No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1106270
American Medical Association (AMA)
Park, Miseon& Mitchell, Wilfrid J.& Rafii, Fatemeh. Effect of Trehalose and Trehalose Transport on the Tolerance of Clostridium perfringens to Environmental Stress in a Wild Type Strain and Its Fluoroquinolone-Resistant Mutant. International Journal of Microbiology. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1106270
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106270