![](/images/graphics-bg.png)
Optimization of the Medium for the Production of Extracellular Amylase by the Pseudomonas stutzeri ISL B5 Isolated from Municipal Solid Waste
Joint Authors
Dutta, Prajesh
Deb, Akash
Majumdar, Sukanta
Source
International Journal of Microbiology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The management of municipal solid waste is one of the major problems of the present world.
The use of microbial enzymes for sustainable management of the solid waste is the need of the time.
In the present study, we have isolated a potent amylase producing strain (ISL B5) from municipal solid waste.
The strain was identified as Pseudomonas stutzeri (P.
stutzeri) both biochemically and by 16S rDNA sequencing.
The optimization studies revealed that the strain ISL B5 exhibited maximum activity in the liquid media containing 2% starch (2.77 U/ml), 0.8% peptone (2.77 U/ml), and 0.001% Ca2+ ion (2.49 U/ml) under the pH 7.5 (2.59 U/ml), temperature 40°C (2.63 U/ml), and 25 h of incubation period (2.49 U/ml).
The highest activity of crude enzyme has also been optimized at the pH 8 (2.49 U/ml).
American Psychological Association (APA)
Dutta, Prajesh& Deb, Akash& Majumdar, Sukanta. 2016. Optimization of the Medium for the Production of Extracellular Amylase by the Pseudomonas stutzeri ISL B5 Isolated from Municipal Solid Waste. International Journal of Microbiology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106271
Modern Language Association (MLA)
Dutta, Prajesh…[et al.]. Optimization of the Medium for the Production of Extracellular Amylase by the Pseudomonas stutzeri ISL B5 Isolated from Municipal Solid Waste. International Journal of Microbiology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1106271
American Medical Association (AMA)
Dutta, Prajesh& Deb, Akash& Majumdar, Sukanta. Optimization of the Medium for the Production of Extracellular Amylase by the Pseudomonas stutzeri ISL B5 Isolated from Municipal Solid Waste. International Journal of Microbiology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106271
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106271