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Synergistic Interaction of Methanol Extract from Canarium odontophyllum Miq. Leaf in Combination with Oxacillin against Methicillin-Resistant Staphylococcus aureus (MRSA) ATCC 33591
Joint Authors
Basri, Dayang Fredalina
Sandra, Vimashiinee
Source
International Journal of Microbiology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-23
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Canarium odontophyllum (CO) Miq.
has been considered as one of the most sought-after plant species in Sarawak, Malaysia, due to its nutritional and pharmacological benefits.
This study aimed to evaluate the pharmacodynamic interaction of crude methanol and acetone extracts from CO leaves in combination with oxacillin, vancomycin, and linezolid, respectively, against MRSA ATCC 33591 as preliminary study has reported its potential antistaphylococcal activity.
The broth microdilution assay revealed that both methanol and acetone extracts were bactericidal with Minimum Inhibitory Concentration (MIC) of 312.5 μg/mL and 156.25 μg/mL and Minimum Bactericidal Concentration (MBC) of 625 μg/mL and 312.5 μg/mL, respectively.
Fractional Inhibitory Concentration (FIC) indices were obtained via the chequerboard dilution assay where methanol extract-oxacillin, acetone extract-oxacillin, methanol extract-linezolid, and acetone extract-linezolid combinations exhibited synergism (FIC index ≤ 0.5).
The synergistic action of the methanol extract-oxacillin combination was verified by time-kill analysis where bactericidal effect was observed at concentration of 1/8 × MIC of both compounds at 9.6 h compared to oxacillin alone.
As such, these findings postulated that both extracts exert their anti-MRSA mechanism of action similar to that of vancomycin and provide evidence that the leaves of C.
odontophyllum have the potential to be developed into antistaphylococcal agents.
American Psychological Association (APA)
Basri, Dayang Fredalina& Sandra, Vimashiinee. 2016. Synergistic Interaction of Methanol Extract from Canarium odontophyllum Miq. Leaf in Combination with Oxacillin against Methicillin-Resistant Staphylococcus aureus (MRSA) ATCC 33591. International Journal of Microbiology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106273
Modern Language Association (MLA)
Basri, Dayang Fredalina& Sandra, Vimashiinee. Synergistic Interaction of Methanol Extract from Canarium odontophyllum Miq. Leaf in Combination with Oxacillin against Methicillin-Resistant Staphylococcus aureus (MRSA) ATCC 33591. International Journal of Microbiology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1106273
American Medical Association (AMA)
Basri, Dayang Fredalina& Sandra, Vimashiinee. Synergistic Interaction of Methanol Extract from Canarium odontophyllum Miq. Leaf in Combination with Oxacillin against Methicillin-Resistant Staphylococcus aureus (MRSA) ATCC 33591. International Journal of Microbiology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106273
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106273