Modulation of mecA Gene Expression by Essential Oil from Salvia sclarea and Synergism with Oxacillin in Methicillin Resistant Staphylococcus epidermidis Carrying Different Types of Staphylococcal Chromosomal Cassette mec
Joint Authors
Mikulášová, Mária
Vaverková, Štefánia
Chovanová, Romana
Source
International Journal of Microbiology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-06
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The essential oil (EO) from Salvia sclarea was shown to increase the susceptibility of methicillin resistant Staphylococcus epidermidis (MRSE) isolates to oxacillin.
The purpose of this study was to investigate the effect of EO from S.
sclarea on expression of mecA gene of MRSE carrying different types of staphylococcal chromosomal cassette (SCCmec) and to evaluate potential synergistic effect of EO with oxacillin.
Using real-time PCR we found that EO alone inhibited the expression of the resistant genes mecA, mecR1, and mecI and blaZ, blaR1, and blaI.
The use of the combination of EO with oxacillin resulted in significantly inhibited expression of mecA gene in all tested strains with different types of SCCmec.
Using time-kill assay and checkerboard assay we confirmed synergistic effect of EO from S.
sclarea and oxacillin in MRSE.
American Psychological Association (APA)
Chovanová, Romana& Mikulášová, Mária& Vaverková, Štefánia. 2016. Modulation of mecA Gene Expression by Essential Oil from Salvia sclarea and Synergism with Oxacillin in Methicillin Resistant Staphylococcus epidermidis Carrying Different Types of Staphylococcal Chromosomal Cassette mec. International Journal of Microbiology،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1106279
Modern Language Association (MLA)
Chovanová, Romana…[et al.]. Modulation of mecA Gene Expression by Essential Oil from Salvia sclarea and Synergism with Oxacillin in Methicillin Resistant Staphylococcus epidermidis Carrying Different Types of Staphylococcal Chromosomal Cassette mec. International Journal of Microbiology No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1106279
American Medical Association (AMA)
Chovanová, Romana& Mikulášová, Mária& Vaverková, Štefánia. Modulation of mecA Gene Expression by Essential Oil from Salvia sclarea and Synergism with Oxacillin in Methicillin Resistant Staphylococcus epidermidis Carrying Different Types of Staphylococcal Chromosomal Cassette mec. International Journal of Microbiology. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1106279
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106279
