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Hypoxia Associated Proteolytic Processing of OS-9 by the Metalloproteinase Meprin β
Joint Authors
Martin, Barry Lee
Conley, Sabena Michelle
Harris, Regine Simone
Stanley, Corshe Devon
Niyitegeka, Jean-Marie Vianney
Ongeri, Elimelda Moige
Source
International Journal of Nephrology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-13
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Meprin metalloproteases play a role in the pathology of ischemia/reperfusion- (IR-) induced renal injury.
The endoplasmic reticulum-associated protein, osteosarcoma-9 (OS-9), has been shown to interact with the carboxyl-terminal tail of meprin β.
More importantly, OS-9 interacts with the hypoxia inducible factor-1α (HIF-1α) and the prolyl-hydroxylase, proteins which mediate the cell’s response to hypoxia.
To determine if OS-9 is a meprin substrate, kidney proteins from meprin αβ knockout mice (αβKO) (which lack endogenous meprins) and purified human OS-9 were incubated with activated forms of meprin A and meprin B, and Western blot analysis was used to evaluate proteolytic processing of OS-9.
Fragmentation of OS-9 was observed in reactions with meprin B, but not meprin A.
To determine whether meprin B cleaves OS-9 in vivo, wild-type (WT) and meprin αβKO mice were subjected to IR-induced renal injury.
Fragmentation of OS-9 was observed in kidney proteins from WT mice subjected to IR, but not in meprin αβKO counterparts.
Transfection of kidney cells (MDCK and HEK293) with meprin β cDNA prevented accumulation of OS-9 following exposure to the hypoxia mimic, CoCl2.
These data suggest that meprin β interaction with OS-9 plays a role in the hypoxia response associated with IR-induced renal injury.
American Psychological Association (APA)
Martin, Barry Lee& Conley, Sabena Michelle& Harris, Regine Simone& Stanley, Corshe Devon& Niyitegeka, Jean-Marie Vianney& Ongeri, Elimelda Moige. 2016. Hypoxia Associated Proteolytic Processing of OS-9 by the Metalloproteinase Meprin β. International Journal of Nephrology،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1106341
Modern Language Association (MLA)
Martin, Barry Lee…[et al.]. Hypoxia Associated Proteolytic Processing of OS-9 by the Metalloproteinase Meprin β. International Journal of Nephrology No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1106341
American Medical Association (AMA)
Martin, Barry Lee& Conley, Sabena Michelle& Harris, Regine Simone& Stanley, Corshe Devon& Niyitegeka, Jean-Marie Vianney& Ongeri, Elimelda Moige. Hypoxia Associated Proteolytic Processing of OS-9 by the Metalloproteinase Meprin β. International Journal of Nephrology. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1106341
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106341