Optimization of Process Parameters for ε-Polylysine Production by Response Surface Methods

Abstract EN

ε-Polylysine (ε-PL) is a highly safe natural food preservative with a broad antimicrobial spectrum, excellent corrosion resistances, and great commercial potentials.

In the present work, we evaluated the ε-PL adsorption performances of HZB-3B and D155 resins and optimized the adsorption and desorption conditions by single-factor test, response surface method, and orthogonal design.

The complexes of resin and ε-PL were characterized by SEM and FITR.

The results indicated that D155 resin had the best ε-PL adsorption performance and was selected for the separation and purification of ε-PL.

The conditions for the static adsorption of ε-PL on D155 resin were optimized as follows: ε-PL solution 40 g/L, pH 8.5, resins 15 g/L, and absorption time 14 h.

The adsorption efficiency of ε-PL under the optimal conditions was 96.84%.

The ε-PL adsorbed on the D155 resin was easily desorbed with 0.4 mol/L HCl at 30°C in 10 h.

The highest desorption efficiency was 97.57% and the overall recovery of ε-PL was 94.49% under the optimal conditions.

The excellent ε-PL adsorption and desorption properties of D155 resin including high selectivity and adsorption capacity, easy desorption, and high stability make it a good candidate for the isolation of ε-PL from fermentation broths.