Development and Validation of an UHPLC-QqQ-MS Technique for Simultaneous Determination of Ten Bioactive Components in Fangji Huangqi Tang
Joint Authors
Wang, Xiaoli
Liu, Xiao
Zhu, Tingting
Cai, Baochang
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of this study is to develop an ultrahigh performance liquid chromatography method coupled with triple quadrupole mass spectrometry for simultaneous determination of tetrandrine, fangchinoline, atractylenolide I, atractylenolide III, calycosin-7-O-β-D-glucoside, glycyrrhizin, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin in Fangji Huangqi Tang (FHT).
The chromatographic separation was performed on a reversed-C18 column, eluted with a mixture of 0.1% acetic acid and acetonitrile at 0.4 mL/min.
The separation of these ten compounds was achieved by linear gradient elution.
The method was strictly validated with respect to specificity, precision, accuracy, and repeatability.
All the compounds showed good linearities (r≥0.999).
The LOQs of the ten components were 0.36, 0.18, 0.09, 0.43, 0.02, 1.89, 0.26, 0.18, 0.61, and 0.48 ng/mL for tetrandrine, fangchinoline, atractylenolide I, atractylenolide III, calycosin-7-O-β-D-glucoside, glycyrrhizin, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin, respectively.
The LODs of the ten components were 0.11, 0.05, 0.03, 0.13, 0.01, 0.57, 0.08, 0.05, 0.18, and 0.14 ng/mL for tetrandrine, fangchinoline, atractylenolide I, atractylenolide III, calycosin-7-O-β-D-glucoside, glycyrrhizin, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin, respectively.
The method was proven to be specific and reliable, which would provide a meaningful basis for the quality control and evaluation of FHT during its clinical application.
American Psychological Association (APA)
Wang, Xiaoli& Liu, Xiao& Zhu, Tingting& Cai, Baochang. 2016. Development and Validation of an UHPLC-QqQ-MS Technique for Simultaneous Determination of Ten Bioactive Components in Fangji Huangqi Tang. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1107265
Modern Language Association (MLA)
Wang, Xiaoli…[et al.]. Development and Validation of an UHPLC-QqQ-MS Technique for Simultaneous Determination of Ten Bioactive Components in Fangji Huangqi Tang. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1107265
American Medical Association (AMA)
Wang, Xiaoli& Liu, Xiao& Zhu, Tingting& Cai, Baochang. Development and Validation of an UHPLC-QqQ-MS Technique for Simultaneous Determination of Ten Bioactive Components in Fangji Huangqi Tang. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1107265
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107265