Comparative Study of Novel Ratio Spectra and Isoabsorptive Point Based Spectrophotometric Methods: Application on a Binary Mixture of Ascorbic Acid and Rutin
Joint Authors
Darwish, Hany W.
Bakheit, Ahmed H.
Naguib, Ibrahim A.
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
This paper presents novel methods for spectrophotometric determination of ascorbic acid (AA) in presence of rutin (RU) (coformulated drug) in their combined pharmaceutical formulation.
The seven methods are ratio difference (RD), isoabsorptive_RD (Iso_RD), amplitude summation (A_Sum), isoabsorptive point, first derivative of the ratio spectra (1DD), mean centering (MCN), and ratio subtraction (RS).
On the other hand, RU was determined directly by measuring the absorbance at 358 nm in addition to the two novel Iso_RD and A_Sum methods.
The work introduced in this paper aims to compare these different methods, showing the advantages for each and making a comparison of analysis results.
The calibration curve is linear over the concentration range of 4–50 μg/mL for AA and RU.
The results show the high performance of proposed methods for the analysis of the binary mixture.
The optimum assay conditions were established and the proposed methods were successfully applied for the assay of the two drugs in laboratory prepared mixtures and combined pharmaceutical tablets with excellent recoveries.
No interference was observed from common pharmaceutical additives.
American Psychological Association (APA)
Darwish, Hany W.& Bakheit, Ahmed H.& Naguib, Ibrahim A.. 2016. Comparative Study of Novel Ratio Spectra and Isoabsorptive Point Based Spectrophotometric Methods: Application on a Binary Mixture of Ascorbic Acid and Rutin. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1107280
Modern Language Association (MLA)
Darwish, Hany W.…[et al.]. Comparative Study of Novel Ratio Spectra and Isoabsorptive Point Based Spectrophotometric Methods: Application on a Binary Mixture of Ascorbic Acid and Rutin. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1107280
American Medical Association (AMA)
Darwish, Hany W.& Bakheit, Ahmed H.& Naguib, Ibrahim A.. Comparative Study of Novel Ratio Spectra and Isoabsorptive Point Based Spectrophotometric Methods: Application on a Binary Mixture of Ascorbic Acid and Rutin. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1107280
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107280