Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides
Author
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-12
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Biotinylation of deoxyguanosine at an abasic site in double-stranded oligodeoxynucleotides was studied.
The biotinylation of deoxyguanosine is achieved by copper-catalyzed click reaction after the conjugation of the oligodeoxynucleotide with 2-oxohex-5-ynal.
The biotinylation enables visualization of the biotinylated oligodeoxynucleotides by chemiluminescence on a nylon membrane.
In order to investigate the biotinylated site, the biotinylated oligodeoxynucleotides were amplified by the DNA polymerase chain reaction.
Replacement of guanine opposing the abasic site with adenine generated by the activity of the terminal deoxynucleotidyl transferase of DNA polymerase was detected by DNA sequencing analysis and restriction endonuclease digestion.
This study suggests that 2-oxohex-5-ynal may be useful for the detection of the unpaired deoxyguanosine endogenously generated at abasic sites in genomic DNA.
American Psychological Association (APA)
Wu, Chun. 2016. Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-4.
https://search.emarefa.net/detail/BIM-1107317
Modern Language Association (MLA)
Wu, Chun. Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-4.
https://search.emarefa.net/detail/BIM-1107317
American Medical Association (AMA)
Wu, Chun. Biotinylation of Deoxyguanosine at the Abasic Site in Double-Stranded Oligodeoxynucleotides. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-4.
https://search.emarefa.net/detail/BIM-1107317
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107317