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Analysis of Flavonoid in Medicinal Plant Extract Using Infrared Spectroscopy and Chemometrics
Joint Authors
Wulandari, Lestyo
Retnaningtyas, Yuni
Nuri, Yuni
Lukman, Hilmia
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-26
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Infrared (IR) spectroscopy combined with chemometrics has been developed for simple analysis of flavonoid in the medicinal plant extract.
Flavonoid was extracted from medicinal plant leaves by ultrasonication and maceration.
IR spectra of selected medicinal plant extract were correlated with flavonoid content using chemometrics.
The chemometric method used for calibration analysis was Partial Last Square (PLS) and the methods used for classification analysis were Linear Discriminant Analysis (LDA), Soft Independent Modelling of Class Analogies (SIMCA), and Support Vector Machines (SVM).
In this study, the calibration of NIR model that showed best calibration with R2 and RMSEC value was 0.9916499 and 2.1521897, respectively, while the accuracy of all classification models (LDA, SIMCA, and SVM) was 100%.
R2 and RMSEC of calibration of FTIR model were 0.8653689 and 8.8958149, respectively, while the accuracy of LDA, SIMCA, and SVM was 86.0%, 91.2%, and 77.3%, respectively.
PLS and LDA of NIR models were further used to predict unknown flavonoid content in commercial samples.
Using these models, the significance of flavonoid content that has been measured by NIR and UV-Vis spectrophotometry was evaluated with paired samples t-test.
The flavonoid content that has been measured with both methods gave no significant difference.
American Psychological Association (APA)
Wulandari, Lestyo& Retnaningtyas, Yuni& Nuri, Yuni& Lukman, Hilmia. 2016. Analysis of Flavonoid in Medicinal Plant Extract Using Infrared Spectroscopy and Chemometrics. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1107318
Modern Language Association (MLA)
Wulandari, Lestyo…[et al.]. Analysis of Flavonoid in Medicinal Plant Extract Using Infrared Spectroscopy and Chemometrics. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1107318
American Medical Association (AMA)
Wulandari, Lestyo& Retnaningtyas, Yuni& Nuri, Yuni& Lukman, Hilmia. Analysis of Flavonoid in Medicinal Plant Extract Using Infrared Spectroscopy and Chemometrics. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1107318
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107318