Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering
Joint Authors
Ren, Hairui
Chen, Zhaoyang
Zhang, Xin
Zhao, Yongmei
Wang, Zheng
Wu, Zhenglong
Xu, Haijun
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Concentrations of S-Adenosyl-L-Methionine (SAM) in aqueous solution and fermentation liquids were quantitatively determined by surface-enhanced Raman scattering (SERS) and verified by high-pressure liquid chromatography (HPLC).
The Ag nanoparticle/silicon nanowire array substrate was fabricated and employed as an active SERS substrate to indirectly measure the SAM concentration.
The linear relationship between the integrated intensity of peak centered at ~2920 cm−1 in SERS spectra and the SAM concentration was established, and the limit of detections of SAM concentrations was analyzed to be ~0.1 g/L.
The concentration of SAM in real solution could be predicted by the linear relationship and verified by the HPLC detection method.
The relative deviations (δ) of the predicted SAM concentration are less than 13% and the correlation coefficient is 0.9998.
Rolling-Circle Filter was utilized to subtract fluorescence background and the optimal results were obtained when the radius of the analyzing circle is 650 cm−1.
American Psychological Association (APA)
Ren, Hairui& Chen, Zhaoyang& Zhang, Xin& Zhao, Yongmei& Wang, Zheng& Wu, Zhenglong…[et al.]. 2016. Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1107320
Modern Language Association (MLA)
Ren, Hairui…[et al.]. Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1107320
American Medical Association (AMA)
Ren, Hairui& Chen, Zhaoyang& Zhang, Xin& Zhao, Yongmei& Wang, Zheng& Wu, Zhenglong…[et al.]. Rapid and Quantitative Determination of S-Adenosyl-L-Methionine in the Fermentation Process by Surface-Enhanced Raman Scattering. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1107320
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107320