Improved Chiral Separation of (R,S)-Goitrin by SFC: An Application in Traditional Chinese Medicine
Joint Authors
Ma, Shuang-Cheng
Dai, Zhong
Nie, Li-xing
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-21
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Like chemical drugs, research and development of herbal medicine also have a need to resolve enantiomers.
To help illustrating the antiviral bioactivity of Isatidis Radix, a widely used traditional Chinese medicine (TCM), supercritical fluid chromatography (SFC) was used for analytical and preparative separation of (R,S)-goitrin, which was reported as the active ingredient of the herbal.
Improved resolution was achieved on Chiralpak IC column, using acetonitrile as the organic modifier, representing a tenfold increase in speed, compared to the previous normal phase HPLC (NPLC) method.
The newly developed chromatographic method was validated in terms of linearity, precision, limit of detection (LOD), and limit of quantitation (LOQ).
Scale-up purification of (R)-goitrin and (S)-goitrin was performed on a preparative column with >90% total recovery.
The absolute stereochemical assignment of the purified isomers was determined through optical rotation study.
This attempt explored SFC’s application in chiral research of traditional Chinese medicine.
American Psychological Association (APA)
Nie, Li-xing& Dai, Zhong& Ma, Shuang-Cheng. 2016. Improved Chiral Separation of (R,S)-Goitrin by SFC: An Application in Traditional Chinese Medicine. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1107341
Modern Language Association (MLA)
Nie, Li-xing…[et al.]. Improved Chiral Separation of (R,S)-Goitrin by SFC: An Application in Traditional Chinese Medicine. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1107341
American Medical Association (AMA)
Nie, Li-xing& Dai, Zhong& Ma, Shuang-Cheng. Improved Chiral Separation of (R,S)-Goitrin by SFC: An Application in Traditional Chinese Medicine. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1107341
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107341