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Determination of the Residual Anthracene Concentration in Cultures of Haloalkalitolerant Actinomycetes by Excitation Fluorescence, Emission Fluorescence, and Synchronous Fluorescence: Comparative Study
Joint Authors
Ramírez Durán, Ninfa
Gómez-Oliván, Leobardo Manuel
Lara-Severino, Reyna del Carmen
Camacho-López, Miguel Ángel
García-Macedo, Jessica Marlene
Sandoval-Trujillo, Ángel H.
Isaac-Olive, Keila
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Polycyclic aromatic hydrocarbons (PAHs) are compounds that can be quantified by fluorescence due to their high quantum yield.
Haloalkalitolerant bacteria tolerate wide concentration ranges of NaCl and pH.
They are potentially useful in the PAHs bioremediation of saline environments.
However, it is known that salinity of the sample affects fluorescence signal regardless of the method.
The objective of this work was to carry out a comparative study based on the sensitivity, linearity, and detection limits of the excitation, emission, and synchronous fluorescence methods, during the quantification of the residual anthracene concentration from the following haloalkalitolerant actinomycetes cultures Kocuria rosea, Kocuria palustris, Microbacterium testaceum, and 4 strains of Nocardia farcinica, in order to establish the proper fluorescence method to study the PAHs biodegrading capacity of haloalkalitolerant actinobacteria.
The study demonstrated statistical differences among the strains and among the fluorescence methods regarding the anthracene residual concentration.
The results showed that excitation and emission fluorescence methods performed very similarly but sensitivity in excitation fluorescence is slightly higher.
Synchronous fluorescence using Δλ=150 nm is not the most convenient method.
Therefore we propose the excitation fluorescence as the fluorescence method to be used in the study of the PAHs biodegrading capacity of haloalkalitolerant actinomycetes.
American Psychological Association (APA)
Lara-Severino, Reyna del Carmen& Camacho-López, Miguel Ángel& García-Macedo, Jessica Marlene& Gómez-Oliván, Leobardo Manuel& Sandoval-Trujillo, Ángel H.& Isaac-Olive, Keila…[et al.]. 2016. Determination of the Residual Anthracene Concentration in Cultures of Haloalkalitolerant Actinomycetes by Excitation Fluorescence, Emission Fluorescence, and Synchronous Fluorescence: Comparative Study. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1107345
Modern Language Association (MLA)
Lara-Severino, Reyna del Carmen…[et al.]. Determination of the Residual Anthracene Concentration in Cultures of Haloalkalitolerant Actinomycetes by Excitation Fluorescence, Emission Fluorescence, and Synchronous Fluorescence: Comparative Study. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1107345
American Medical Association (AMA)
Lara-Severino, Reyna del Carmen& Camacho-López, Miguel Ángel& García-Macedo, Jessica Marlene& Gómez-Oliván, Leobardo Manuel& Sandoval-Trujillo, Ángel H.& Isaac-Olive, Keila…[et al.]. Determination of the Residual Anthracene Concentration in Cultures of Haloalkalitolerant Actinomycetes by Excitation Fluorescence, Emission Fluorescence, and Synchronous Fluorescence: Comparative Study. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1107345
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107345