Catalase-Based Modified Graphite Electrode for Hydrogen Peroxide Detection in Different Beverages
Joint Authors
Antiochia, Riccarda
Bollella, Paolo
Favero, Gabriele
Mazzei, Franco
Fusco, Giovanni
Tortolini, Cristina
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-18
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
A catalase-based (NAF/MWCNTs) nanocomposite film modified glassy carbon electrode for hydrogen peroxide (H2O2) detection was developed.
The developed biosensor was characterized in terms of its bioelectrochemical properties.
Cyclic voltammetry (CV) technique was employed to study the redox features of the enzyme in the absence and in the presence of nanomaterials dispersed in Nafion® polymeric solution.
The electron transfer coefficient, α, and the electron transfer rate constant, ks, were found to be 0.42 and 1.71 s−1, at pH 7.0, respectively.
Subsequently, the same modification steps were applied to mesoporous graphite screen-printed electrodes.
Also, these electrodes were characterized in terms of their main electrochemical and kinetic parameters.
The biosensor performances improved considerably after modification with nanomaterials.
Moreover, the association of Nafion with carbon nanotubes retained the biological activity of the redox protein.
The enzyme electrode response was linear in the range 2.5–1150 μmol L−1, with LOD of 0.83 μmol L−1.
From the experimental data, we can assess the possibility of using the modified biosensor as a useful tool for H2O2 determination in packaged beverages.
American Psychological Association (APA)
Fusco, Giovanni& Bollella, Paolo& Mazzei, Franco& Favero, Gabriele& Antiochia, Riccarda& Tortolini, Cristina. 2016. Catalase-Based Modified Graphite Electrode for Hydrogen Peroxide Detection in Different Beverages. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1107375
Modern Language Association (MLA)
Fusco, Giovanni…[et al.]. Catalase-Based Modified Graphite Electrode for Hydrogen Peroxide Detection in Different Beverages. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1107375
American Medical Association (AMA)
Fusco, Giovanni& Bollella, Paolo& Mazzei, Franco& Favero, Gabriele& Antiochia, Riccarda& Tortolini, Cristina. Catalase-Based Modified Graphite Electrode for Hydrogen Peroxide Detection in Different Beverages. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1107375
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107375