Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX
Joint Authors
Tian, Rui-Yun
Lin, Chao
Yu, Shi-Yu
Gong, Sheng
Hu, Pan
Li, Yan-Song
Wu, Zong-Cheng
Gao, Yang
Zhou, Yu
Liu, Zeng-Shan
Ren, Hong-Lin
Lu, Shi-Ying
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The existing assays for detecting brevetoxin (BTX) depend on expensive equipment with a professional operator or on an antibody with limited stability, which requires complex processes, a high cost, and a considerable amount of time.
The development of an alternative detection probe is another promising research direction.
This paper reports the use of aptamers binding to BTX-2 in an analytical assay using the systematic evolution of ligands by exponential enrichment (SELEX).
After 12 rounds of selection, the secondary structures of 25 sequences were predicted.
Compared to other aptamers, Bap5 has relatively high affinity with the lowest dissociation constant of 4.83 μM, and IC50 is 73.81 ng mL−1.
A good linear regression formula of y=30.688x-7.329 with a coefficient correlation of R2 = 0.9798 was obtained using a biotin-avidin ELISA.
Moreover, there is no cross-reaction with the detected marine toxins, except for BTX-2.
Thus, Bap5 has potential to detect BTX-2 in shellfish in the future as a substitute for the recognition probe.
American Psychological Association (APA)
Tian, Rui-Yun& Lin, Chao& Yu, Shi-Yu& Gong, Sheng& Hu, Pan& Li, Yan-Song…[et al.]. 2016. Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX. Journal of Analytical Methods in Chemistry،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1107393
Modern Language Association (MLA)
Tian, Rui-Yun…[et al.]. Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX. Journal of Analytical Methods in Chemistry No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1107393
American Medical Association (AMA)
Tian, Rui-Yun& Lin, Chao& Yu, Shi-Yu& Gong, Sheng& Hu, Pan& Li, Yan-Song…[et al.]. Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX. Journal of Analytical Methods in Chemistry. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1107393
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107393