Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC
Joint Authors
Ai, Yujie
Yu, Zhi
Chen, Yuqiong
Zhu, Xiaojing
Ai, Zeyi
Liu, Shuyuan
Ni, Dejiang
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-05
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A pre-column derivatization high-performance liquid chromatography (HPLC) method was developed and optimized to characterize and quantify the monosaccharides present in tea polysaccharides (TPS) isolated from Yingshuang green tea.
TPS sample was hydrolyzed with trifluoroacetic acid, subjected to pre-column derivatization using 1-phenyl-3-methyl-5-pyrazolone (PMP), and separated on an Agilent TC-C18 column (4.6 mm × 250 mm, 5 μm) with UV detection at 250 nm.
A mixture of ten PMP derivatives of standard monosaccharides (mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose, arabinose, and fucose) could be baseline separated within 20 min.
Moreover, quantitative analysis of the component monosaccharides in Yingshuang green tea TPS was achieved, indicating the TPS consisted of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose, and arabinose in the molar contents of 0.72, 0.78, 0.89, 0.13, 0.15, 0.36, 0.39, 0.36, 0.36, and 0.38 μM, respectively.
Recovery efficiency for component monosaccharides from TPS ranged from 93.6 to 102.4% with RSD values lower than 2.5%.
In conclusion, pre-column derivatization HPLC provides a rapid, reproducible, accurate, and quantitative method for analysis of the monosaccharide composition and contents in TPS, which may help to further explore the relationship between TPS monosaccharides isolated from different tea varieties and their biological activity.
American Psychological Association (APA)
Ai, Yujie& Yu, Zhi& Chen, Yuqiong& Zhu, Xiaojing& Ai, Zeyi& Liu, Shuyuan…[et al.]. 2016. Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC. Journal of Chemistry،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1107714
Modern Language Association (MLA)
Ai, Yujie…[et al.]. Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC. Journal of Chemistry No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1107714
American Medical Association (AMA)
Ai, Yujie& Yu, Zhi& Chen, Yuqiong& Zhu, Xiaojing& Ai, Zeyi& Liu, Shuyuan…[et al.]. Rapid Determination of the Monosaccharide Composition and Contents in Tea Polysaccharides from Yingshuang Green Tea by Pre-Column Derivatization HPLC. Journal of Chemistry. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1107714
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107714