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Binding Orientations and Lipid Interactions of Human Amylin at Zwitterionic and Anionic Lipid Bilayers
Joint Authors
Qian, Zhenyu
Jia, Yan
Wei, Guanghong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Increasing evidence suggests that the interaction of human islet amyloid polypeptide (hIAPP) with lipids may facilitate hIAPP aggregation and cause the death of pancreatic islet β-cells.
However, the detailed hIAPP-membrane interactions and the influences of lipid compositions are unclear.
In this study, as a first step to understand the mechanism of membrane-mediated hIAPP aggregation, we investigate the binding behaviors of hIAPP monomer at zwitterionic palmitoyloleoyl-phosphatidylcholine (POPC) bilayer by performing atomistic molecular dynamics simulations.
The results are compared with those of hIAPP at anionic palmitoyloleoyl-phosphatidylglycerol (POPG) bilayers.
We find that the adsorption of hIAPP to POPC bilayer is mainly initiated from the C-terminal region and the peptide adopts a helical structure with multiple binding orientations, while the adsorption to POPG bilayer is mostly initiated from the N-terminal region and hIAPP displays one preferential binding orientation, with its hydrophobic residues exposed to water.
hIAPP monomer inserts into POPC lipid bilayers more readily than into POPG bilayers.
Peptide-lipid interaction analyses show that the different binding features of hIAPP at POPC and POPG bilayers are attributed to different magnitudes of electrostatic and hydrogen-bonding interactions with lipids.
This study provides mechanistic insights into the different interaction behaviors of hIAPP with zwitterionic and anionic lipid bilayers.
American Psychological Association (APA)
Qian, Zhenyu& Jia, Yan& Wei, Guanghong. 2015. Binding Orientations and Lipid Interactions of Human Amylin at Zwitterionic and Anionic Lipid Bilayers. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1107981
Modern Language Association (MLA)
Qian, Zhenyu…[et al.]. Binding Orientations and Lipid Interactions of Human Amylin at Zwitterionic and Anionic Lipid Bilayers. Journal of Diabetes Research No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1107981
American Medical Association (AMA)
Qian, Zhenyu& Jia, Yan& Wei, Guanghong. Binding Orientations and Lipid Interactions of Human Amylin at Zwitterionic and Anionic Lipid Bilayers. Journal of Diabetes Research. 2015. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1107981
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107981