Increased Autoreactivity of the Complement-Activating Molecule Mannan-Binding Lectin in a Type 1 Diabetes Model
Joint Authors
Thiel, Steffen
Ruseva, Marieta Milkova
Malik, Talat Habib
Hoffmann-Petersen, Ingeborg Torp
Pickering, Matthew Caleb
Hansen, Troels Krarup
Østergaard, Jakob Appel
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-10
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Diabetic kidney disease is the leading cause of end-stage renal failure despite intensive treatment of modifiable risk factors.
Identification of new drug targets is therefore of paramount importance.
The complement system is emerging as a potential new target.
The lectin pathway of the complement system, initiated by the carbohydrate-recognition molecule mannan-binding lectin (MBL), is linked to poor kidney prognosis in diabetes.
We hypothesized that MBL activates complement upon binding within the diabetic glomerulus.
Methods.
We investigated this by comparing complement deposition and activation in kidneys from streptozotocin-induced diabetic mice and healthy control mice.
Results.
After 20 weeks of diabetes, glomerular deposition of MBL was significantly increased.
Diabetic animals had 2.0-fold higher (95% CI 1.6–2.5) immunofluorescence intensity from anti-MBL antibodies compared with controls ( P < 0.001 ).
Diabetes and control groups did not differ in glomerular immunofluorescence intensity obtained by antibodies against complement factors C4, C3, and C9.
However, the circulating complement activation product C3a was increased in diabetes as compared to control mice ( P = 0.04 ).
Conclusion.
20 weeks of diabetes increased MBL autoreactivity in the kidney and circulating C3a concentration.
Together with previous findings, these results indicate direct effects of MBL within the kidney in diabetes.
American Psychological Association (APA)
Østergaard, Jakob Appel& Ruseva, Marieta Milkova& Malik, Talat Habib& Hoffmann-Petersen, Ingeborg Torp& Pickering, Matthew Caleb& Thiel, Steffen…[et al.]. 2016. Increased Autoreactivity of the Complement-Activating Molecule Mannan-Binding Lectin in a Type 1 Diabetes Model. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107985
Modern Language Association (MLA)
Østergaard, Jakob Appel…[et al.]. Increased Autoreactivity of the Complement-Activating Molecule Mannan-Binding Lectin in a Type 1 Diabetes Model. Journal of Diabetes Research No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1107985
American Medical Association (AMA)
Østergaard, Jakob Appel& Ruseva, Marieta Milkova& Malik, Talat Habib& Hoffmann-Petersen, Ingeborg Torp& Pickering, Matthew Caleb& Thiel, Steffen…[et al.]. Increased Autoreactivity of the Complement-Activating Molecule Mannan-Binding Lectin in a Type 1 Diabetes Model. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107985
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107985