miRNA-375 a Sensor of Glucotoxicity Is Altered in the Serum of Children with Newly Diagnosed Type 1 Diabetes
Joint Authors
Marchand, Lucien
Jalabert, Audrey
Meugnier, Emmanuelle
Van den Hende, Kathleen
Fabien, Nicole
Nicolino, Marc
Madec, Anne-Marie
Thivolet, Charles
Rome, Sophie
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-24
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
The use of miRNAs as biomarkers for Type 1 Diabetes (T1D) risk is attractive as T1D is usually diagnosed in front of acute symptoms.
As miR-375 is highly expressed in the endocrine pancreas, we postulated that its circulating level might reflect beta cell alterations and might be altered in the blood of T1D patients recently diagnosed.
Methods.
Sera were obtained from 22 T1D children at onset of the disease, before subcutaneous insulin treatment, and from 10 nondiabetic pediatric controls.
MiR-375 seric level was quantified by stem-loop RT-PCR-based assay.
MiRNAs regulations in isolated human islets in response to high glucose concentrations were determined by TaqMan Low-Density Array.
Results.
The abundance of miR-375, among the 410 miRNAs detected in human islets, mirrored its well-established role in rodent islet biology.
Upregulated miRNAs targeted genes involved in islet homeostasis and regulation of beta cell mass.
Downregulated miRNAs, including miR-375, were involved in pancreas secretion and protein turnover.
Seric level of miR-375 was lower in T1D children versus age-matched controls, without any correlations with HbA1c, glycaemia, and number of autoantibodies.
Conclusion.
Altered circulating level of miR-375 at onset of T1D might be a general biomarker of metabolic alterations and inflammation associated with the disease.
American Psychological Association (APA)
Marchand, Lucien& Jalabert, Audrey& Meugnier, Emmanuelle& Van den Hende, Kathleen& Fabien, Nicole& Nicolino, Marc…[et al.]. 2016. miRNA-375 a Sensor of Glucotoxicity Is Altered in the Serum of Children with Newly Diagnosed Type 1 Diabetes. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107990
Modern Language Association (MLA)
Marchand, Lucien…[et al.]. miRNA-375 a Sensor of Glucotoxicity Is Altered in the Serum of Children with Newly Diagnosed Type 1 Diabetes. Journal of Diabetes Research No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1107990
American Medical Association (AMA)
Marchand, Lucien& Jalabert, Audrey& Meugnier, Emmanuelle& Van den Hende, Kathleen& Fabien, Nicole& Nicolino, Marc…[et al.]. miRNA-375 a Sensor of Glucotoxicity Is Altered in the Serum of Children with Newly Diagnosed Type 1 Diabetes. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1107990
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107990