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Epigenetic Studies Point to DNA ReplicationRepair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes
Joint Authors
Sarras, Michael Peter
Leontovich, Alexey A.
Intine, Robert V.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Metabolic memory (MM) is defined as the persistence of diabetic (DM) complications even after glycemic control is pharmacologically achieved.
Using a zebrafish diabetic model that induces a MM state, we previously reported that, in this model, tissue dysfunction was of a heritable nature based on cell proliferation studies in limb tissue and this correlated with epigenetic DNA methylation changes that paralleled alterations in gene expression.
In the current study, control, DM, and MM excised fin tissues were further analyzed by MeDIP sequencing and microarray techniques.
Bioinformatics analysis of the data found that genes of the DNA replication/DNA metabolism process group (with upregulation of the apex1, mcm2, mcm4, orc3, lig1, and dnmt1 genes) were altered in the DM state and these molecular changes continued into MM.
Interestingly, DNA methylation changes could be found as far as 6–13 kb upstream of the transcription start site for these genes suggesting potential higher levels of epigenetic control.
In conclusion, DNA methylation changes in members of the DNA replication/repair process group best explain the heritable nature of cell proliferation impairment found in the zebrafish DM/MM model.
These results are consistent with human diabetic epigenetic studies and provide one explanation for the persistence of long term tissue complications as seen in diabetes.
American Psychological Association (APA)
Leontovich, Alexey A.& Intine, Robert V.& Sarras, Michael Peter. 2016. Epigenetic Studies Point to DNA ReplicationRepair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108034
Modern Language Association (MLA)
Leontovich, Alexey A.…[et al.]. Epigenetic Studies Point to DNA ReplicationRepair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes. Journal of Diabetes Research No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1108034
American Medical Association (AMA)
Leontovich, Alexey A.& Intine, Robert V.& Sarras, Michael Peter. Epigenetic Studies Point to DNA ReplicationRepair Genes as a Basis for the Heritable Nature of Long Term Complications in Diabetes. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108034
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108034