Involvement of Histone Lysine Methylation in p21 Gene Expression in Rat Kidney In Vivo and Rat Mesangial Cells In Vitro under Diabetic Conditions
Joint Authors
Sun, Guang-dong
Han, Hongbo
Li, Chaoyuan
Liu, Shujun
Li, Xiangjun
Li, Xiaoxia
Cui, Peihe
Li, Qifeng
Guo, Qiaoyan
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-29
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Diabetic nephropathy (DN), a common complication associated with type 1 and type 2 diabetes mellitus (DM), characterized by glomerular mesangial expansion, inflammation, accumulation of extracellular matrix (ECM) protein, and hypertrophy, is the major cause of end-stage renal disease (ESRD).
Increasing evidence suggested that p21-dependent glomerular and mesangial cell (MC) hypertrophy play key roles in the pathogenesis of DN.
Recently, posttranscriptional modifications (PTMs) have uncovered novel molecular mechanisms involved in DN.
However, precise regulatory mechanism of histone lysine methylation (HKme) mediating p21 related hypertrophy associated with DN is not clear.
We evaluated the roles of HKme and histone methyltransferase (HMT) SET7/9 in p21 gene expression in glomeruli of diabetic rats and in high glucose- (HG-) treated rat mesangial cells (RMCs).
p21 gene expression was upregulated in diabetic rats glomeruli; chromatin immunoprecipitation (ChIP) assays showed decreased histone H3-lysine9-dimethylation (H3K9me2) accompanied with enhanced histone H3-lysine4-methylation (H3K4me1/3) and SET7/9 occupancies at the p21 promoter.
HG-treated RMCs exhibited increased p21 mRNA, H3K4me level, SET7/9 recruitment, and inverse H3K9me, which were reversed by TGF-β1 antibody.
These data uncovered key roles of H3Kme and SET7/9 responsible for p21 gene expression in vivo and in vitro under diabetic conditions and confirmed preventive effect of TGF-β1 antibody on DN.
American Psychological Association (APA)
Li, Xiangjun& Li, Chaoyuan& Li, Xiaoxia& Cui, Peihe& Li, Qifeng& Guo, Qiaoyan…[et al.]. 2016. Involvement of Histone Lysine Methylation in p21 Gene Expression in Rat Kidney In Vivo and Rat Mesangial Cells In Vitro under Diabetic Conditions. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1108083
Modern Language Association (MLA)
Li, Xiangjun…[et al.]. Involvement of Histone Lysine Methylation in p21 Gene Expression in Rat Kidney In Vivo and Rat Mesangial Cells In Vitro under Diabetic Conditions. Journal of Diabetes Research No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1108083
American Medical Association (AMA)
Li, Xiangjun& Li, Chaoyuan& Li, Xiaoxia& Cui, Peihe& Li, Qifeng& Guo, Qiaoyan…[et al.]. Involvement of Histone Lysine Methylation in p21 Gene Expression in Rat Kidney In Vivo and Rat Mesangial Cells In Vitro under Diabetic Conditions. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1108083
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108083