Exenatide Activates the APPL1-AMPK-PPARα Axis to Prevent Diabetic Cardiomyocyte Apoptosis
Joint Authors
Lei, Xiaotian
QiNan, Wu
XiaGuang, Gan
WuQuan, Deng
ZiWen, Liang
Chen, Bing
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
To investigate the effect and mechanism of the exenatide on diabetic cardiomyopathy.
Methods.
Rats were divided into control group, diabetes group (D), diabetes treated with insulin (DI) group, and diabetes treat with exenatide (DE) group.
We detected apoptosis rate by TUNEL, the adiponectin and high molecular weight adiponectin (HMW-adiponectin) by ELISA, and the expression of APPL1, p-AMPK/T-AMPK, PPARα, and NF-κB by immunohistochemistry and western blotting.
Results.
Compared with the D group, the apoptosis in the Control and DE groups was decreased ( P < 0.05 ); the adiponectin and HMW-adiponectin were increased ( P < 0.05 ); the APPL1, p-AMPK/T-AMPK, PPARα, and LV −dP/dt were increased ( P < 0.05 ); and the NF-κB, GRP78, and LVEDP were decreased ( P < 0.05 ).
Compared with DE group, the glucose levels in the DI group were similar ( P < 0.05 ); the apoptosis and LVEDP were increased; the APPL1, p-AMPK/T-AMPK, PPARα, and LV −dP/dt were decreased ( P < 0.05 ); the NF-κB and GRP78 were increased ( P < 0.05 ); the adiponectin and HMW-adiponectin were significantly decreased ( P < 0.05 ).
Conclusion.
Our model of diabetic cardiomyopathy was constructed successfully.
After being treated with exenatide, the adiponectin and HMW-adiponectin and the APPL1-AMPK-PPARα axis were increased, the NF-κB and the apoptosis were decreased, the cardiac function of the diabetic rats was improved, and these effects were independent of glucose control.
American Psychological Association (APA)
Lei, Xiaotian& QiNan, Wu& XiaGuang, Gan& WuQuan, Deng& Chen, Bing& ZiWen, Liang. 2015. Exenatide Activates the APPL1-AMPK-PPARα Axis to Prevent Diabetic Cardiomyocyte Apoptosis. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108105
Modern Language Association (MLA)
Lei, Xiaotian…[et al.]. Exenatide Activates the APPL1-AMPK-PPARα Axis to Prevent Diabetic Cardiomyocyte Apoptosis. Journal of Diabetes Research No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1108105
American Medical Association (AMA)
Lei, Xiaotian& QiNan, Wu& XiaGuang, Gan& WuQuan, Deng& Chen, Bing& ZiWen, Liang. Exenatide Activates the APPL1-AMPK-PPARα Axis to Prevent Diabetic Cardiomyocyte Apoptosis. Journal of Diabetes Research. 2015. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108105
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108105