Collagen V Is a Potential Substrate for Clostridial Collagenase G in Pancreatic Islet Isolation
Joint Authors
Igarashi, Kazuhiko
Shima, Hiroki
Inagaki, Akiko
Imura, Takehiro
Yamagata, Youhei
Watanabe, Kimiko
Goto, Masafumi
Murayama, Kazutaka
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The clostridial collagenases, H and G, play key roles in pancreatic islet isolation.
Collagenases digest the peptide bond between Yaa and the subsequent Gly in Gly-Xaa-Yaa repeats.
To fully understand the pancreatic islet isolation process, identification of the collagenase substrates in the tissue is very important.
Although collagen types I and III were reported as possible substrates for collagenase H, the substrate for collagenase G remains unknown.
In this study, collagen type V was focused upon as the target for collagenases.
In vitro digestion experiments for collagen type V were performed and analyzed by SDS-PAGE and mass spectrometry.
Porcine pancreatic tissues were digested in vitro under three conditions and observed during digestion.
The results revealed that collagen type V was only digested by collagenase G and that the digestion was initiated from the N-terminal part.
Tissue degradation during porcine islet isolation was only observed in the presence of both collagenases H and G.
These findings suggest that collagen type V is one of the substrates for collagenase G.
The enzymatic activity of collagenase G appears to be more important for pancreatic islet isolation in large mammals such as pigs and humans.
American Psychological Association (APA)
Shima, Hiroki& Inagaki, Akiko& Imura, Takehiro& Yamagata, Youhei& Watanabe, Kimiko& Igarashi, Kazuhiko…[et al.]. 2016. Collagen V Is a Potential Substrate for Clostridial Collagenase G in Pancreatic Islet Isolation. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108114
Modern Language Association (MLA)
Shima, Hiroki…[et al.]. Collagen V Is a Potential Substrate for Clostridial Collagenase G in Pancreatic Islet Isolation. Journal of Diabetes Research No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1108114
American Medical Association (AMA)
Shima, Hiroki& Inagaki, Akiko& Imura, Takehiro& Yamagata, Youhei& Watanabe, Kimiko& Igarashi, Kazuhiko…[et al.]. Collagen V Is a Potential Substrate for Clostridial Collagenase G in Pancreatic Islet Isolation. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108114
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108114