Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro
Joint Authors
Lv, Zhimei
Hu, Mengsi
Ren, Xiaoxu
Fan, Minghua
Zhen, Junhui
Chen, Liqun
Lin, Jiangong
Ding, Nannan
Wang, Qun
Wang, Rong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-06
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Fyn, a member of the Src family of tyrosine kinases, is a key regulator in cytoskeletal remodeling in a variety of cell types.
Recent studies have demonstrated that Fyn is responsible for nephrin tyrosine phosphorylation, which will result in polymerization of actin filaments and podocyte damage.
Thus detailed involvement of Fyn in podocytes is to be elucidated.
In this study, we investigated the potential role of Fyn/ROCK signaling and its interactions with paxillin.
Our results presented that high glucose led to filamentous actin (F-actin) rearrangement in podocytes, accompanied by paxillin phosphorylation and increased cell motility, during which Fyn and ROCK were markedly activated.
Gene knockdown of Fyn by siRNA showed a reversal effect on high glucose-induced podocyte damage and ROCK activation; however, inhibition of ROCK had no significant effects on Fyn phosphorylation.
These observations demonstrate that in vitro Fyn mediates high glucose-induced actin cytoskeleton remodeling of podocytes via promoting ROCK activation and paxillin phosphorylation.
American Psychological Association (APA)
Lv, Zhimei& Hu, Mengsi& Ren, Xiaoxu& Fan, Minghua& Zhen, Junhui& Chen, Liqun…[et al.]. 2016. Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1108164
Modern Language Association (MLA)
Lv, Zhimei…[et al.]. Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro. Journal of Diabetes Research No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1108164
American Medical Association (AMA)
Lv, Zhimei& Hu, Mengsi& Ren, Xiaoxu& Fan, Minghua& Zhen, Junhui& Chen, Liqun…[et al.]. Fyn Mediates High Glucose-Induced Actin Cytoskeleton Reorganization of Podocytes via Promoting ROCK Activation In Vitro. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1108164
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108164