Urinary Extracellular Vesicles: Potential Biomarkers of Renal Function in Diabetic Patients
Joint Authors
Kuśnierz-Cabala, Beata
Kuźniewski, Marek
Gala-Błądzińska, Agnieszka
Kamińska, Agnieszka
Platt, Mark
Kasprzyk, Joanna
Woźnicka, Olga
Jany, Benedykt R.
Krok, Franciszek
Piekoszewski, Wojciech
Stępień, Ewa Ł.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The aim of this study was to check the relationship between the density of urinary EVs, their size distribution, and the progress of early renal damage in type 2 diabetic patients (DMt2).
Patients were enrolled to this study, and glycated hemoglobin (HbA1c) below 7% was a threshold for properly controlled diabetic patients (CD) and poorly controlled diabetic patients (UD).
Patients were further divided into two groups: diabetic patients without renal failure (NRF) and with renal failure (RF) according to the Glomerular Filtration Rate.
Density and diameter of EVs were determined by Tunable Resistive Pulse Sensing.
Additionally, EVs were visualized by means of Transmission and Environmental Scanning Electron Microscopy.
Nano-liquid chromatography coupled offline with mass spectrometry (MALDI-TOF-MS/MS) was applied for proteomic analysis.
RF had reduced density of EVs compared to NRF.
The size distribution study showed that CD had larger EVs (mode) than UD (115 versus 109 nm; p<0.05); nevertheless the mean EVs diameter was smaller in controls than in the CD group (123 versus 134 nm; p<0.05).
It was demonstrated that EVs are abundant in urine.
Albumin, uromodulin, and number of unique proteins related to cell stress and secretion were detected in the EVs fraction.
Density and size of urinary EVs reflect deteriorated renal function and can be considered as potential renal damage biomarkers.
American Psychological Association (APA)
Kamińska, Agnieszka& Platt, Mark& Kasprzyk, Joanna& Kuśnierz-Cabala, Beata& Gala-Błądzińska, Agnieszka& Woźnicka, Olga…[et al.]. 2016. Urinary Extracellular Vesicles: Potential Biomarkers of Renal Function in Diabetic Patients. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1108168
Modern Language Association (MLA)
Kamińska, Agnieszka…[et al.]. Urinary Extracellular Vesicles: Potential Biomarkers of Renal Function in Diabetic Patients. Journal of Diabetes Research No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1108168
American Medical Association (AMA)
Kamińska, Agnieszka& Platt, Mark& Kasprzyk, Joanna& Kuśnierz-Cabala, Beata& Gala-Błądzińska, Agnieszka& Woźnicka, Olga…[et al.]. Urinary Extracellular Vesicles: Potential Biomarkers of Renal Function in Diabetic Patients. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1108168
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108168