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In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications
Joint Authors
Orlando, Giuseppe
Niu, Guoguang
McQuilling, John P.
Zhou, Yu
Opara, Emmanuel C.
Soker, Shay
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
β-Cell replacement through transplantation is the only curative treatment to establish a long-term stable euglycemia in diabetic patients.
Owing to the shortage of donor tissue, attempts are being made to develop alternative sources of insulin-secreting cells.
Stem cells differentiation and reprograming as well as isolating pancreatic progenitors from different sources are some examples; however, no approach has yet yielded a clinically relevant solution.
Dissociated islet cells that are cultured in cell numbers by in vitro proliferation provide a promising platform for redifferentiation towards β-cells phenotype.
In this study, we cultured islet-derived cells in vitro and examined the expression of β-cell genes during the proliferation.
Islets were isolated from porcine pancreases and enzymatically digested to dissociate the component cells.
The cells proliferated well in tissue culture plates and were subcultured for no more than 5 passages.
Only 10% of insulin expression, as measured by PCR, was preserved in each passage.
High glucose media enhanced insulin expression by about 4–18 fold, suggesting a glucose-dependent effect in the proliferated islet-derived cells.
The islet-derived cells also expressed other pancreatic genes such as Pdx1, NeuroD, glucagon, and somatostatin.
Taken together, these results indicate that pancreatic islet-derived cells, proliferated in vitro, retained the expression capacity for key pancreatic genes, thus suggesting that the cells may be redifferentiated into insulin-secreting β-like cells.
American Psychological Association (APA)
Niu, Guoguang& McQuilling, John P.& Zhou, Yu& Opara, Emmanuel C.& Orlando, Giuseppe& Soker, Shay. 2016. In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108171
Modern Language Association (MLA)
Niu, Guoguang…[et al.]. In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications. Journal of Diabetes Research No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1108171
American Medical Association (AMA)
Niu, Guoguang& McQuilling, John P.& Zhou, Yu& Opara, Emmanuel C.& Orlando, Giuseppe& Soker, Shay. In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108171
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108171