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Sulfatide Preserves Insulin Crystals Not by Being Integrated in the Lattice but by Stabilizing Their Surface
Joint Authors
Buschard, K.
Bracey, Austin W.
McElroy, Daniel L.
Magis, Andrew T.
Osterbye, Thomas
Atkinson, Mark A.
Bailey, Kate M.
Posgai, Amanda L.
Ostrov, David A.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-14
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Background.
Sulfatide is known to chaperone insulin crystallization within the pancreatic beta cell, but it is not known if this results from sulfatide being integrated inside the crystal structure or by binding the surface of the crystal.
With this study, we aimed to characterize the molecular mechanisms underlying the integral role for sulfatide in stabilizing insulin crystals prior to exocytosis.
Methods.
We cocrystallized human insulin in the presence of sulfatide and solved the structure by molecular replacement.
Results.
The crystal structure of insulin crystallized in the presence of sulfatide does not reveal ordered occupancy representing sulfatide in the crystal lattice, suggesting that sulfatide does not permeate the crystal lattice but exerts its stabilizing effect by alternative interactions such as on the external surface of insulin crystals.
Conclusions.
Sulfatide is known to stabilize insulin crystals, and we demonstrate here that in beta cells sulfatide is likely coating insulin crystals.
However, there is no evidence for sulfatide to be built into the crystal lattice.
American Psychological Association (APA)
Buschard, K.& Bracey, Austin W.& McElroy, Daniel L.& Magis, Andrew T.& Osterbye, Thomas& Atkinson, Mark A.…[et al.]. 2016. Sulfatide Preserves Insulin Crystals Not by Being Integrated in the Lattice but by Stabilizing Their Surface. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-4.
https://search.emarefa.net/detail/BIM-1108190
Modern Language Association (MLA)
Buschard, K.…[et al.]. Sulfatide Preserves Insulin Crystals Not by Being Integrated in the Lattice but by Stabilizing Their Surface. Journal of Diabetes Research No. 2016 (2016), pp.1-4.
https://search.emarefa.net/detail/BIM-1108190
American Medical Association (AMA)
Buschard, K.& Bracey, Austin W.& McElroy, Daniel L.& Magis, Andrew T.& Osterbye, Thomas& Atkinson, Mark A.…[et al.]. Sulfatide Preserves Insulin Crystals Not by Being Integrated in the Lattice but by Stabilizing Their Surface. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-4.
https://search.emarefa.net/detail/BIM-1108190
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108190