Development of Diagnostic Biomarkers for Detecting Diabetic Retinopathy at Early Stages Using Quantitative Proteomics
Joint Authors
Yu, Hyeong Gon
Kim, Youngsoo
Park, Taesung
Min, Hophil
Kim, Sang Jin
Oh, Sohee
Kim, Kyunggon
Jin, Jonghwa
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-22, 22 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-09
Country of Publication
Egypt
No. of Pages
22
Main Subjects
Abstract EN
Diabetic retinopathy (DR) is a common microvascular complication caused by diabetes mellitus (DM) and is a leading cause of vision impairment and loss among adults.
Here, we performed a comprehensive proteomic analysis to discover biomarkers for DR.
First, to identify biomarker candidates that are specifically expressed in human vitreous, we performed data-mining on both previously published DR-related studies and our experimental data; 96 proteins were then selected.
To confirm and validate the selected biomarker candidates, candidates were selected, confirmed, and validated using plasma from diabetic patients without DR (No DR) and diabetics with mild or moderate nonproliferative diabetic retinopathy (Mi or Mo NPDR) using semiquantitative multiple reaction monitoring (SQ-MRM) and stable-isotope dilution multiple reaction monitoring (SID-MRM).
Additionally, we performed a multiplex assay using 15 biomarker candidates identified in the SID-MRM analysis, which resulted in merged AUC values of 0.99 (No DR versus Mo NPDR) and 0.93 (No DR versus Mi and Mo NPDR).
Although further validation with a larger sample size is needed, the 4-protein marker panel (APO4, C7, CLU, and ITIH2) could represent a useful multibiomarker model for detecting the early stages of DR.
American Psychological Association (APA)
Jin, Jonghwa& Min, Hophil& Kim, Sang Jin& Oh, Sohee& Kim, Kyunggon& Yu, Hyeong Gon…[et al.]. 2015. Development of Diagnostic Biomarkers for Detecting Diabetic Retinopathy at Early Stages Using Quantitative Proteomics. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-22.
https://search.emarefa.net/detail/BIM-1108207
Modern Language Association (MLA)
Jin, Jonghwa…[et al.]. Development of Diagnostic Biomarkers for Detecting Diabetic Retinopathy at Early Stages Using Quantitative Proteomics. Journal of Diabetes Research No. 2016 (2016), pp.1-22.
https://search.emarefa.net/detail/BIM-1108207
American Medical Association (AMA)
Jin, Jonghwa& Min, Hophil& Kim, Sang Jin& Oh, Sohee& Kim, Kyunggon& Yu, Hyeong Gon…[et al.]. Development of Diagnostic Biomarkers for Detecting Diabetic Retinopathy at Early Stages Using Quantitative Proteomics. Journal of Diabetes Research. 2015. Vol. 2016, no. 2016, pp.1-22.
https://search.emarefa.net/detail/BIM-1108207
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108207