Islet Stellate Cells Isolated from Fibrotic Islet of Goto-Kakizaki Rats Affect Biological Behavior of Beta-Cell
Joint Authors
Li, Fengfei
Chen, Bijun
Sun, Zilin
Li, Wei
Li, Ling
Zha, Min
Zhou, S.
Bachem, M. G.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
We previously isolated islet stellate cells (ISCs) from healthy Wistar rat islets.
In the present study, we isolated “already primed by diabetic environment” ISCs from islets of Goto-Kakizaki rats, determined the gene profile of these cells, and assessed the effects of these ISCs on beta-cell function and survival.
We detected gene expression of ISCs by digital gene expression.
INS-1 cell proliferation, apoptosis, and insulin production were measured after being treated with ISCs supernatant (SN).
We observed the similar expression pattern of ISCs and PSCs, but 1067 differentially expressed genes.
Insulin production in INS-1 cells cultured with ISC-SN was significantly reduced.
The 5-ethynyl-2′-deoxyuridine-positive INS-1 cells treated with ISC-SN were decreased.
Propidium iodide- (PI-) positive INS-1 cells were 2.6-fold higher than those in control groups.
Caspase-3 activity was increased.
In conclusion, ISCs presented in fibrotic islet of GK rats might be special PSCs, which impaired beta-cell function and proliferation and increased beta-cell apoptosis.
American Psychological Association (APA)
Li, Fengfei& Chen, Bijun& Li, Wei& Li, Ling& Zha, Min& Zhou, S.…[et al.]. 2015. Islet Stellate Cells Isolated from Fibrotic Islet of Goto-Kakizaki Rats Affect Biological Behavior of Beta-Cell. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1108219
Modern Language Association (MLA)
Li, Fengfei…[et al.]. Islet Stellate Cells Isolated from Fibrotic Islet of Goto-Kakizaki Rats Affect Biological Behavior of Beta-Cell. Journal of Diabetes Research No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1108219
American Medical Association (AMA)
Li, Fengfei& Chen, Bijun& Li, Wei& Li, Ling& Zha, Min& Zhou, S.…[et al.]. Islet Stellate Cells Isolated from Fibrotic Islet of Goto-Kakizaki Rats Affect Biological Behavior of Beta-Cell. Journal of Diabetes Research. 2015. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1108219
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108219