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High Glucose and Lipopolysaccharide Prime NLRP3 Inflammasome via ROSTXNIP Pathway in Mesangial Cells
Joint Authors
Yang, Maojun
Long, Yang
Feng, Hong
Gu, Junling
Zhou, Xueqin
Liu, Shuang
Lü, Shishi
Luo, Qiaoyan
Gou, Fang
Xu, Yong
Chen, Guo
Huang, Wei
Gao, Chenlin
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-05
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
While inflammation is considered a central component in the development in diabetic nephropathy, the mechanism remains unclear.
The NLRP3 inflammasome acts as both a sensor and a regulator of the inflammatory response.
The NLRP3 inflammasome responds to exogenous and endogenous danger signals, resulting in cleavage of procaspase-1 and activation of cytokines IL-1β, IL-18, and IL-33, ultimately triggering an inflammatory cascade reaction.
This study observed the expression of NLRP3 inflammasome signaling stimulated by high glucose, lipopolysaccharide, and reactive oxygen species (ROS) inhibitor N-acetyl-L-cysteine in glomerular mesangial cells, aiming to elucidate the mechanism by which the NLRP3 inflammasome signaling pathway may contribute to diabetic nephropathy.
We found that the expression of thioredoxin-interacting protein (TXNIP), NLRP3, and IL-1β was observed by immunohistochemistry in vivo.
Simultaneously, the mRNA and protein levels of TXNIP, NLRP3, procaspase-1, and IL-1β were significantly induced by high glucose concentration and lipopolysaccharide in a dose-dependent and time-dependent manner in vitro.
This induction by both high glucose and lipopolysaccharide was significantly inhibited by N-acetyl-L-cysteine.
Our results firstly reveal that high glucose and lipopolysaccharide activate ROS/TXNIP/ NLRP3/IL-1β inflammasome signaling in glomerular mesangial cells, suggesting a mechanism by which inflammation may contribute to the development of diabetic nephropathy.
American Psychological Association (APA)
Feng, Hong& Gu, Junling& Gou, Fang& Huang, Wei& Gao, Chenlin& Chen, Guo…[et al.]. 2016. High Glucose and Lipopolysaccharide Prime NLRP3 Inflammasome via ROSTXNIP Pathway in Mesangial Cells. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108223
Modern Language Association (MLA)
Feng, Hong…[et al.]. High Glucose and Lipopolysaccharide Prime NLRP3 Inflammasome via ROSTXNIP Pathway in Mesangial Cells. Journal of Diabetes Research No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1108223
American Medical Association (AMA)
Feng, Hong& Gu, Junling& Gou, Fang& Huang, Wei& Gao, Chenlin& Chen, Guo…[et al.]. High Glucose and Lipopolysaccharide Prime NLRP3 Inflammasome via ROSTXNIP Pathway in Mesangial Cells. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108223
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108223