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miRNAs in Urine Extracellular Vesicles as Predictors of Early-Stage Diabetic Nephropathy
Joint Authors
Zheng, Zongji
Xue, Yaoming
Guan, Meiping
Jia, Yijie
Zhang, Qian
Tang, Chuan
Xu, Wenwei
Xiao, Zhizhou
Wang, Ling
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
miR-192, miR-194, and miR-215 are enriched in the kidney and play roles in the pathogenesis of diabetic nephropathy (DN).
Extracellular vesicles (EVs) can be detected in body fluids and may serve as disease biomarkers.
Methods.
Eighty type 2 diabetes patients with normoalbuminuria ( n = 30 ), microalbuminuria ( n = 30 ), or macroalbuminuria ( n = 20 ), as well as 10 healthy controls, were enrolled in this study.
Real-time PCR was used to evaluate urinary EV miRNAs expression.
Results.
The miR-192 levels were significantly higher than the miR-194 and miR-215 levels in urine EVs and all three miRNAs were significantly increased in the microalbuminuric group compared with the normoalbuminuric and control subjects but were decreased in the macroalbuminuric group.
In patients with normoalbuminuria and microalbuminuria, miR-192 was positively correlated with albuminuria ( r = 0 .
357 , P = 0.0 05 ) levels and transforming growth factor- (TGF-) β1 ( r = 0 .
356 , P = 0.0 05 ) expression.
Receiver operating characteristic (ROC) curve analysis revealed that miR-192 was better than miR-194 and miR-215 in discriminating the normoalbuminuric group from the microalbuminuric group.
Exposure of human renal tubular epithelial cells to high glucose increased the expression of both miRNAs in cellular supernatant EVs, indicating a potential source.
Conclusion.
These results suggest the potential use of urinary EV miR-192 as a biomarker of the early stage of DN.
American Psychological Association (APA)
Jia, Yijie& Guan, Meiping& Zheng, Zongji& Zhang, Qian& Tang, Chuan& Xu, Wenwei…[et al.]. 2016. miRNAs in Urine Extracellular Vesicles as Predictors of Early-Stage Diabetic Nephropathy. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108254
Modern Language Association (MLA)
Jia, Yijie…[et al.]. miRNAs in Urine Extracellular Vesicles as Predictors of Early-Stage Diabetic Nephropathy. Journal of Diabetes Research No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1108254
American Medical Association (AMA)
Jia, Yijie& Guan, Meiping& Zheng, Zongji& Zhang, Qian& Tang, Chuan& Xu, Wenwei…[et al.]. miRNAs in Urine Extracellular Vesicles as Predictors of Early-Stage Diabetic Nephropathy. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108254
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108254