Inability of Some Commercial Assays to Measure Suppression of Glucagon Secretion
Joint Authors
Wewer Albrechtsen, Nicolai J.
Veedfald, Simon
Plamboeck, Astrid
Deacon, Carolyn F.
Hartmann, Bolette
Knop, Filip K.
Vilsboll, Tina
Holst, Jens J.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-29
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Glucagon levels are increasingly being included as endpoints in clinical study design and more than 400 current diabetes-related clinical trials have glucagon as an outcome measure.
The reliability of immune-based technologies used to measure endogenous glucagon concentrations is, therefore, important.
We studied the ability of immunoassays based on four different technologies to detect changes in levels of glucagon under conditions where glucagon levels are strongly suppressed.
To our surprise, the most advanced technological methods, employing electrochemiluminescence or homogeneous time resolved fluorescence (HTRF) detection, were not capable of detecting the suppression induced by a glucose clamp (6 mmol/L) with or without atropine in five healthy male participants, whereas a radioimmunoassay and a spectrophotometry-based ELISA were.
In summary, measurement of glucagon is challenging even when state-of-the-art immune-based technologies are used.
Clinical researchers using glucagon as outcome measures may need to reconsider the validity of their chosen glucagon assay.
The current study demonstrates that the most advanced approach is not necessarily the best when measuring a low-abundant peptide such as glucagon in humans.
American Psychological Association (APA)
Wewer Albrechtsen, Nicolai J.& Veedfald, Simon& Plamboeck, Astrid& Deacon, Carolyn F.& Hartmann, Bolette& Knop, Filip K.…[et al.]. 2015. Inability of Some Commercial Assays to Measure Suppression of Glucagon Secretion. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1108279
Modern Language Association (MLA)
Wewer Albrechtsen, Nicolai J.…[et al.]. Inability of Some Commercial Assays to Measure Suppression of Glucagon Secretion. Journal of Diabetes Research No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1108279
American Medical Association (AMA)
Wewer Albrechtsen, Nicolai J.& Veedfald, Simon& Plamboeck, Astrid& Deacon, Carolyn F.& Hartmann, Bolette& Knop, Filip K.…[et al.]. Inability of Some Commercial Assays to Measure Suppression of Glucagon Secretion. Journal of Diabetes Research. 2015. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1108279
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108279