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Glucose Tolerance, MTHFR C677T and NOS3 G894T Polymorphisms, and Global DNA Methylation in Mixed Ancestry African Individuals
Joint Authors
Erasmus, R. T.
Matsha, T. E.
Pheiffer, Carmen
Mutize, Tinashe
Kengne, Andre Pascal
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of this study is to quantify global DNA methylation and investigate the relationship with diabetes status and polymorphisms in MTHFR C677T and NOS3 G894T genes in mixed ancestry subjects from South Africa.
Global DNA methylation was measured, and MTHFR rs1801133 and NOS3 rs1799983 polymorphisms were genotyped using high throughput real-time polymerase chain reaction and direct DNA sequencing.
Of the 564 participants, 158 (28%) individuals had T2DM of which 97 (17.2%) were screen-detected cases.
Another 119 (21.1%) had prediabetes, that is, impaired fasting glucose, impaired glucose tolerance, or the combination of both, and the remainder 287 (50.9%) had normal glucose tolerance.
Global DNA methylation was significantly higher in prediabetes and screen-detected diabetes than in normal glucose tolerance (both p ≤ 0.033 ) and in screen-detected diabetes compared to known diabetes on treatment ( p = 0.019 ).
There was no difference in global DNA methylation between known diabetes on treatment and normal glucose tolerance ( p > 0.999 ).
In multivariable linear regression analysis, only NOS3 was associated with increasing global DNA methylation ( β = 0.943 ; 95% CI: 0.286 to 1.560).
The association of global DNA methylation with screen-detected diabetes but not treated diabetes suggests that glucose control agents to some extent may be reversing DNA methylation.
The association between NOS3 rs1799983 polymorphisms and DNA methylation suggests gene-epigenetic mechanisms through which vascular diabetes complications develop despite adequate metabolic control.
American Psychological Association (APA)
Matsha, T. E.& Pheiffer, Carmen& Mutize, Tinashe& Erasmus, R. T.& Kengne, Andre Pascal. 2016. Glucose Tolerance, MTHFR C677T and NOS3 G894T Polymorphisms, and Global DNA Methylation in Mixed Ancestry African Individuals. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108297
Modern Language Association (MLA)
Matsha, T. E.…[et al.]. Glucose Tolerance, MTHFR C677T and NOS3 G894T Polymorphisms, and Global DNA Methylation in Mixed Ancestry African Individuals. Journal of Diabetes Research No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1108297
American Medical Association (AMA)
Matsha, T. E.& Pheiffer, Carmen& Mutize, Tinashe& Erasmus, R. T.& Kengne, Andre Pascal. Glucose Tolerance, MTHFR C677T and NOS3 G894T Polymorphisms, and Global DNA Methylation in Mixed Ancestry African Individuals. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1108297
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108297