Clopidogrel Protects Endothelium by Hindering TNFα-Induced VCAM-1 Expression through CaMKKβAMPKNrf2 Pathway
Joint Authors
Yang, Huabing
Zhao, Pengjun
Tian, Shiliu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Clopidogrel (INN), an oral antiplatelet drug, has been revealed to have a number of biological properties, for instance, anti-inflammation and antioxidation.
Oxidative stress plays an imperative role in inflammation, diabetes mellitus, atherosclerosis, and cancer.
In the present study, human aortic endothelial cells (HAECs) were employed to explore the anti-inflammatory activity of INN.
INN reduced TNFα-induced reactive oxygen species (ROS) generation and time-dependently prompted the expression and activity of heme oxygenase 1 (HO-1).
Cellular glutathione (GSH) levels were augmented by INN.
shHO-1 blocked the INN suppression of TNFα-induced HL-60 cell adhesion.
The CaMKKβ/AMPK pathway and Nrf2 transcriptional factor were implicated in the induction of HO-1 by INN.
Additionally, TNFα dramatically augmented VCAM-1 expression at protein and mRNA levels.
INN treatment strikingly repressed TNFα-induced expression of VCAM-1 and HL-60 cell adhesion.
Compound C, an AMPK inhibitor, and shNrf2 abolished TNFα-induced expression of VCAM-1 and HL-60 cell adhesion.
Our data suggest that INN diminishes TNFα-stimulated VCAM-1 expression at least in part via HO-1 induction, which is CaMKKβ/AMPK pathway-dependent.
American Psychological Association (APA)
Yang, Huabing& Zhao, Pengjun& Tian, Shiliu. 2015. Clopidogrel Protects Endothelium by Hindering TNFα-Induced VCAM-1 Expression through CaMKKβAMPKNrf2 Pathway. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108315
Modern Language Association (MLA)
Yang, Huabing…[et al.]. Clopidogrel Protects Endothelium by Hindering TNFα-Induced VCAM-1 Expression through CaMKKβAMPKNrf2 Pathway. Journal of Diabetes Research No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1108315
American Medical Association (AMA)
Yang, Huabing& Zhao, Pengjun& Tian, Shiliu. Clopidogrel Protects Endothelium by Hindering TNFα-Induced VCAM-1 Expression through CaMKKβAMPKNrf2 Pathway. Journal of Diabetes Research. 2015. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108315
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108315