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Low-Dose Oxygen Enhances Macrophage-Derived Bacterial Clearance following Cigarette Smoke Exposure
Joint Authors
Bain, William G.
Tripathi, Ashutosh
Mandke, Pooja
Gans, Jonathan H.
D’Alessio, Franco R.
Sidhaye, Venkataramana K.
Aggarwal, Neil R.
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-14
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Background.
Chronic obstructive pulmonary disease (COPD) is a common, smoking-related lung disease.
Patients with COPD frequently suffer disease exacerbations induced by bacterial respiratory infections, suggestive of impaired innate immunity.
Low-dose oxygen is a mainstay of therapy during COPD exacerbations; yet we understand little about whether oxygen can modulate the effects of cigarette smoke on lung immunity.
Methods.
Wild-type mice were exposed to cigarette smoke for 5 weeks, followed by intratracheal instillation of Pseudomonas aeruginosa (PAO1) and 21% or 35–40% oxygen.
After two days, lungs were harvested for PAO1 CFUs, and bronchoalveolar fluid was sampled for inflammatory markers.
In culture, macrophages were exposed to cigarette smoke and oxygen (40%) for 24 hours and then incubated with PAO1, followed by quantification of bacterial phagocytosis and inflammatory markers.
Results.
Mice exposed to 35–40% oxygen after cigarette smoke and PAO1 had improved survival and reduced lung CFUs and inflammation.
Macrophages from these mice expressed less TNF-α and more scavenger receptors.
In culture, macrophages exposed to cigarette smoke and oxygen also demonstrated decreased TNF-α secretion and enhanced phagocytosis of PAO1 bacteria.
Conclusions.
Our findings demonstrate a novel, protective role for low-dose oxygen following cigarette smoke and bacteria exposure that may be mediated by enhanced macrophage phagocytosis.
American Psychological Association (APA)
Bain, William G.& Tripathi, Ashutosh& Mandke, Pooja& Gans, Jonathan H.& D’Alessio, Franco R.& Sidhaye, Venkataramana K.…[et al.]. 2016. Low-Dose Oxygen Enhances Macrophage-Derived Bacterial Clearance following Cigarette Smoke Exposure. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1108704
Modern Language Association (MLA)
Bain, William G.…[et al.]. Low-Dose Oxygen Enhances Macrophage-Derived Bacterial Clearance following Cigarette Smoke Exposure. Journal of Immunology Research No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1108704
American Medical Association (AMA)
Bain, William G.& Tripathi, Ashutosh& Mandke, Pooja& Gans, Jonathan H.& D’Alessio, Franco R.& Sidhaye, Venkataramana K.…[et al.]. Low-Dose Oxygen Enhances Macrophage-Derived Bacterial Clearance following Cigarette Smoke Exposure. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1108704
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108704