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Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response
Joint Authors
Wang, Junhua
Siffert, Myriam
Spiliotis, Markus
Gottstein, Bruno
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-15
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Regulatory T (Tregs) cells play an important role in mediating tolerance to self-antigens but can also mediate detrimental tolerance to tumours and pathogens in a Foxp3-dependent manner.
Genetic tools exploiting the foxp3 locus including bacterial artificial chromosome- (BAC-) transgenic DEpletion of REGulatory T cells (DEREG) mice have provided essential information on Treg biology and the potential therapeutic modulation of tolerance.
In DEREG mice, Foxp3+ Tregs selectively express enhanced green fluorescent protein (eGFP) and diphtheria toxin (DT) receptor, allowing for the specific depletion of Tregs through DT administration.
We here provide a detailed overview about an important consideration that long-term administration of DT induces a humoral immune response with an appropriate production of anti-DT antibodies that can inactivate DT and thus abrogate its effect in the DEREG mouse.
Additionally, we showed that anti-DT mouse serum partially neutralized DT-induced Foxp3 inhibition.
American Psychological Association (APA)
Wang, Junhua& Siffert, Myriam& Spiliotis, Markus& Gottstein, Bruno. 2016. Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1108712
Modern Language Association (MLA)
Wang, Junhua…[et al.]. Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response. Journal of Immunology Research No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1108712
American Medical Association (AMA)
Wang, Junhua& Siffert, Myriam& Spiliotis, Markus& Gottstein, Bruno. Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1108712
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108712