Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée)
Joint Authors
Zhang, Shasha
Hong, Fang
Song, He
Wang, Lei
An, Chunju
Liu, Qi Zhi
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-18
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Insect phenoloxidase (PO) belongs to the type 3 copper protein family and possesses oxidoreductase activities.
PO is typically synthesized as a zymogen called prophenoloxidase (PPO) and requires the proteolytic activation to function.
We here cloned full-length cDNA for 3 previously unidentified PPOs, which we named OfPPO1a, OfPPO1b, and OfPPO3, from Asian corn borer, Ostrinia furnacalis (Gunée), in addition to the previously known OfPPO2.
These conceptual PPOs and OfPPO2 all contain two common copper-binding regions, two potential proteolytic activation sites, a plausible thiol-ester site, and a conserved C-terminal region but lack a secretion signal peptide sequence at the N-terminus.
O.
furnacalis PPOs were highly similar to other insect PPOs (42% to 79% identity) and clustered well with other lepidopteran PPOs.
RT-PCR assay showed the transcripts of the 4 OfPPOs were all detected at the highest level in hemocytes and at the increased amounts after exposure to infection by bacteria and fungi.
Additionally, we established an Escherichia coli (E.
coli) expression system to produce recombinant O.
furnacalis PPO proteins for future use in investigating their functions.
These insights could provide valuable information for better understanding the activation and functioning mechanisms of O.
furnacalis PPOs.
American Psychological Association (APA)
Zhang, Shasha& Hong, Fang& Song, He& Wang, Lei& Liu, Qi Zhi& An, Chunju. 2016. Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée). Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1108723
Modern Language Association (MLA)
Zhang, Shasha…[et al.]. Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée). Journal of Immunology Research No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1108723
American Medical Association (AMA)
Zhang, Shasha& Hong, Fang& Song, He& Wang, Lei& Liu, Qi Zhi& An, Chunju. Cloning, Expression, and Characterization of Prophenoloxidases from Asian Corn Borer, Ostrinia furnacalis (Gunée). Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1108723
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108723