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Effect of HSV-IL12 Loaded Tumor Cell-Based Vaccination in a Mouse Model of High-Grade Neuroblastoma
Joint Authors
Yancey Gillespie, G.
Markert, James M.
Bauer, David F.
Pereboeva, Larisa
Cloud, Gretchen A.
Elzafarany, Osama
Langford, Catherine
Jr., Lawrence S. Lamb
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We designed multimodal tumor vaccine that consists of irradiated tumor cells infected with the oncolytic IL-12-expressing HSV-1 virus, M002.
This vaccine was tested against the syngeneic neuroblastoma mouse model Neuro 2a injected into the right caudate nucleus of the immunocompetent A/J mice.
Mice were vaccinated via intramuscular injection of multimodal vaccine or uninfected irradiated tumor cells at seven and 14 days after tumor establishment.
While there was no survival difference between groups vaccinated with cell-based vaccine applied following tumor injection, a premunition prime/boost vaccination strategy produced a significant survival advantage in both groups and sustained immune response to an intracranial rechallenge of the same tumor.
The syngeneic but unrelated H6 hepatocellular tumor cell line grew unrestricted in vaccinated mice, indicative of vaccine-mediated specific immunity to Neuro 2a tumors.
Longitudinal analyses of tumor-infiltrating lymphocytes revealed a primary adaptive T cell response involving both CD4+ and CD8+ T cell subsets.
Spleen cell mononuclear preparations from vaccinated mice were significantly more cytotoxic to Neuro 2a tumor cells than spleen cells from control mice as demonstrated in a four-hour in vitro cytotoxicity assay.
These results strongly suggest that an irradiated whole cell tumor vaccine incorporating IL-12-expressing M002 HSV can produce a durable, specific immunization in a murine model of intracranial tumor.
American Psychological Association (APA)
Bauer, David F.& Pereboeva, Larisa& Yancey Gillespie, G.& Cloud, Gretchen A.& Elzafarany, Osama& Langford, Catherine…[et al.]. 2016. Effect of HSV-IL12 Loaded Tumor Cell-Based Vaccination in a Mouse Model of High-Grade Neuroblastoma. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108737
Modern Language Association (MLA)
Bauer, David F.…[et al.]. Effect of HSV-IL12 Loaded Tumor Cell-Based Vaccination in a Mouse Model of High-Grade Neuroblastoma. Journal of Immunology Research No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1108737
American Medical Association (AMA)
Bauer, David F.& Pereboeva, Larisa& Yancey Gillespie, G.& Cloud, Gretchen A.& Elzafarany, Osama& Langford, Catherine…[et al.]. Effect of HSV-IL12 Loaded Tumor Cell-Based Vaccination in a Mouse Model of High-Grade Neuroblastoma. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108737
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108737