Immunomodulatory Effects of a Bioactive Compound Isolated from Dryopteris crassirhizoma on the Grass Carp Ctenopharyngodon idella
Joint Authors
Giri, Sib Sankar
Chi, Cheng
Kim, Hyoun Joong
Yun, Saekil
Park, Se Chang
Jun, Jin Woo
Kim, Sang Guen
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In the present study, we investigated effects of compound kaempferol 3-a-L-(4-O-acetyl)rhamnopyranoside-7-a-L-rhamnopyranoside (SA) isolated from Dryopteris crassirhizoma during immune-related gene expression in Ctenopharyngodon idella head kidney macrophages (CIHKM).
The expression of immune-related genes (IL-1β, TNF-α , MyD88, and Mx1) were investigated using real-time PCR at 2 h, 8 h, 12 h, and 24 h after incubation with 1, 10, and 50 μg mL−1 of SA.
Furthermore, fish were injected intraperitoneally with 100 μL of SA, and immune parameters such as lysozyme activity, complement C3, SOD, phagocytic activity, and IgM level were examined at 1, 2, and 3 weeks after injection.
The differential expression of cytokines was observed after exposure to SA.
IL-1β genes displayed significant expression at 2 and 8 h after exposure to 1–10 μg mL−1 of SA.
SA also induced gene expression of cytokines such as MyD88, Mx1, and TNF-α .
Furthermore, enhanced immune parameters in grass carp confirmed the immunomodulatory activity of SA.
Interestingly, this compound has no toxic effect on CIHKM cells as tested by MTT assay.
In addition, fish immunised with 10 μg mL−1 of SA exhibited maximum resistance against Aeromonas hydrophila infection.
These results suggest that SA has the potential to stimulate immune responses in grass carp.
American Psychological Association (APA)
Chi, Cheng& Giri, Sib Sankar& Jun, Jin Woo& Kim, Hyoun Joong& Yun, Saekil& Kim, Sang Guen…[et al.]. 2016. Immunomodulatory Effects of a Bioactive Compound Isolated from Dryopteris crassirhizoma on the Grass Carp Ctenopharyngodon idella. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108753
Modern Language Association (MLA)
Chi, Cheng…[et al.]. Immunomodulatory Effects of a Bioactive Compound Isolated from Dryopteris crassirhizoma on the Grass Carp Ctenopharyngodon idella. Journal of Immunology Research No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1108753
American Medical Association (AMA)
Chi, Cheng& Giri, Sib Sankar& Jun, Jin Woo& Kim, Hyoun Joong& Yun, Saekil& Kim, Sang Guen…[et al.]. Immunomodulatory Effects of a Bioactive Compound Isolated from Dryopteris crassirhizoma on the Grass Carp Ctenopharyngodon idella. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108753
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108753