A Modified NK Cell Degranulation Assay Applicable for Routine Evaluation of NK Cell Function
Joint Authors
Gupta, Maya
Shabrish, Snehal
Madkaikar, Manisha
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-20
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Natural killer (NK) cells play important role in innate immunity against tumors and viral infections.
Studies show that lysosome-associated membrane protein-1 (LAMP-1, CD107a) is a marker for degranulation of NK and cytotoxic T cells and its expression is a sensitive marker for the cytotoxic activity determination.
The conventional methods of determination of CD107a on NK cells involve use of peripheral blood mononuclear cells (PBMC) or pure NK cells and K562 cells as stimulants.
Thus, it requires large volume of blood sample which is usually difficult to obtain in pediatric patients and patients with cytopenia and also requires specialized laboratory for maintaining cell line.
We have designed a flow cytometric assay to determine CD107a on NK cells using whole blood, eliminating the need for isolation of PBMC or isolate NK cells.
This assay uses phorbol-12-myristate-13-acetate (PMA) and calcium ionophore (Ca2+-ionophore) instead of K562 cells for stimulation and thus does not require specialized cell culture laboratory.
CD107a expression on NK cells using modified NK cell degranulation assay compared to the conventional assay was significantly elevated ( p < 0.0001 ).
It was also validated by testing patients diagnosed with familial hemophagocytic lymphohistiocytosis (FHL) with defect in exocytosis.
This assay is rapid, cost effective, and reproducible and requires significantly less volume of blood which is important for clinical evaluation of NK cells.
American Psychological Association (APA)
Shabrish, Snehal& Gupta, Maya& Madkaikar, Manisha. 2016. A Modified NK Cell Degranulation Assay Applicable for Routine Evaluation of NK Cell Function. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1108765
Modern Language Association (MLA)
Shabrish, Snehal…[et al.]. A Modified NK Cell Degranulation Assay Applicable for Routine Evaluation of NK Cell Function. Journal of Immunology Research No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1108765
American Medical Association (AMA)
Shabrish, Snehal& Gupta, Maya& Madkaikar, Manisha. A Modified NK Cell Degranulation Assay Applicable for Routine Evaluation of NK Cell Function. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1108765
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108765