TLR Stimulation Dynamically Regulates Heme and Iron Export Gene Expression in Macrophages
Joint Authors
Philip, Mary
Chiu, Edison Y.
Hajjar, Adeline M.
Abkowitz, Janis L.
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-24
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Pathogenic bacteria have evolved multiple mechanisms to capture iron or iron-containing heme from host tissues or blood.
In response, organisms have developed defense mechanisms to keep iron from pathogens.
Very little of the body’s iron store is available as free heme; rather nearly all body iron is complexed with heme or other proteins.
The feline leukemia virus, subgroup C (FeLV-C) receptor, FLVCR, exports heme from cells.
It was unknown whether FLVCR regulates heme-iron availability after infection, but given that other heme regulatory proteins are upregulated in macrophages in response to bacterial infection, we hypothesized that macrophages dynamically regulate FLVCR.
We stimulated murine primary macrophages or macrophage cell lines with LPS and found that Flvcr is rapidly downregulated in a TLR4/MD2-dependent manner; TLR1/2 and TLR3 stimulation also decreased Flvcr expression.
We identified several candidate TLR-activated transcription factors that can bind to the Flvcr promoter.
Macrophages must balance the need to sequester iron from systemic circulating or intracellular pathogens with the macrophage requirement for heme and iron to produce reactive oxygen species.
Our findings underscore the complexity of this regulation and point to a new role for FLVCR and heme export in macrophages responses to infection and inflammation.
American Psychological Association (APA)
Philip, Mary& Chiu, Edison Y.& Hajjar, Adeline M.& Abkowitz, Janis L.. 2016. TLR Stimulation Dynamically Regulates Heme and Iron Export Gene Expression in Macrophages. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108772
Modern Language Association (MLA)
Philip, Mary…[et al.]. TLR Stimulation Dynamically Regulates Heme and Iron Export Gene Expression in Macrophages. Journal of Immunology Research No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1108772
American Medical Association (AMA)
Philip, Mary& Chiu, Edison Y.& Hajjar, Adeline M.& Abkowitz, Janis L.. TLR Stimulation Dynamically Regulates Heme and Iron Export Gene Expression in Macrophages. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1108772
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108772