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Low Dose BCG Infection as a Model for Macrophage Activation Maintaining Cell Viability
Joint Authors
Chávez-Galán, Leslie
Vesin, Dominique
Martinvalet, Denis
Garcia, Irene
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-19
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Mycobacterium bovis BCG, the current vaccine against tuberculosis, is ingested by macrophages promoting the development of effector functions including cell death and microbicidal mechanisms.
Despite accumulating reports on M.
tuberculosis, mechanisms of BCG/macrophage interaction remain relatively undefined.
In vivo, few bacilli are sufficient to establish a mycobacterial infection; however, in vitro studies systematically use high mycobacterium doses.
In this study, we analyze macrophage/BCG interactions and microenvironment upon infection with low BCG doses and propose an in vitro model to study cell activation without affecting viability.
We show that RAW macrophages infected with BCG at MOI 1 activated higher and sustained levels of proinflammatory cytokines and transcription factors while MOI 0.1 was more efficient for early stimulation of IL-1β, MCP-1, and KC.
Both BCG infection doses induced iNOS and NO in a dose-dependent manner and maintained nuclear and mitochondrial structures.
Microenvironment generated by MOI 1 induced macrophage proliferation but not MOI 0.1 infection.
In conclusion, BCG infection at low dose is an efficient in vitro model to study macrophage/BCG interactions that maintains macrophage viability and mitochondrial structures.
This represents a novel model that can be applied to BCG research fields including mycobacterial infections, cancer immunotherapy, and prevention of autoimmunity and allergies.
American Psychological Association (APA)
Chávez-Galán, Leslie& Vesin, Dominique& Martinvalet, Denis& Garcia, Irene. 2016. Low Dose BCG Infection as a Model for Macrophage Activation Maintaining Cell Viability. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1108774
Modern Language Association (MLA)
Chávez-Galán, Leslie…[et al.]. Low Dose BCG Infection as a Model for Macrophage Activation Maintaining Cell Viability. Journal of Immunology Research No. 2016 (2016), pp.1-17.
https://search.emarefa.net/detail/BIM-1108774
American Medical Association (AMA)
Chávez-Galán, Leslie& Vesin, Dominique& Martinvalet, Denis& Garcia, Irene. Low Dose BCG Infection as a Model for Macrophage Activation Maintaining Cell Viability. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1108774
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108774