![](/images/graphics-bg.png)
Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition
Joint Authors
Breman, Eytan
Ruben, Jurjen M.
Heemskerk, Mirjam H. M.
Roelen, Dave L.
Claas, Frans H.
van Kooten, Cees
Franken, Kees L. M. C.
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In organ transplantation, alloantigens are taken up by antigen presenting cells and presented via the indirect pathway to T-cells which in turn can induce allograft rejection.
Monitoring of these T-cells is of major importance; however no reliable assay is available to routinely monitor indirect allorecognition.
Recently we showed that HLA monomers can be successfully used to monitor indirect allorecognition.
Targeting antigens to endocytic receptors on antigen presenting cells may further enhance the presentation of antigens via HLA class II and improve the efficiency of this assay.
In the current study we explored targeting of HLA monomers to either CD89 expressing monocytes or mannose receptor expressing dendritic cells.
Monomer-antibody complexes were generated using biotin-labeled monomers and avidin labeling of the antibodies.
We demonstrate that targeting the complexes to these receptors resulted in a dose-dependent HLA class II mediated presentation to a T-cell clone.
The immune-complexes were efficiently taken up and presented to T-cells.
However, the level of T-cell reactivity was similar to that when only exogenous antigen was added.
We conclude that HLA-A2 monomers targeted for presentation through CD89 on monocytes or mannose receptor on dendritic cells lead to proper antigen presentation but do not enhance indirect allorecognition via HLA-DR.
American Psychological Association (APA)
Breman, Eytan& Ruben, Jurjen M.& Franken, Kees L. M. C.& Heemskerk, Mirjam H. M.& Roelen, Dave L.& Claas, Frans H.…[et al.]. 2016. Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1108782
Modern Language Association (MLA)
Breman, Eytan…[et al.]. Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition. Journal of Immunology Research No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1108782
American Medical Association (AMA)
Breman, Eytan& Ruben, Jurjen M.& Franken, Kees L. M. C.& Heemskerk, Mirjam H. M.& Roelen, Dave L.& Claas, Frans H.…[et al.]. Uptake of HLA Alloantigens via CD89 and CD206 Does Not Enhance Antigen Presentation by Indirect Allorecognition. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1108782
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108782