The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis
Joint Authors
Tlaskalova-Hogenova, Helena
Heissigerova, Jarmila
Seidler Stangova, Petra
Klimova, Aneta
Svozilkova, Petra
Hrncir, Tomas
Stepankova, Renata
Kverka, Miloslav
Forrester, John V.
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-17
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The microbiota is a crucial modulator of the immune system.
Here, we evaluated how its absence or reduction modifies the inflammatory response in the murine model of experimental autoimmune uveoretinitis (EAU).
We induced EAU in germ-free (GF) or conventionally housed (CV) mice and in CV mice treated with a combination of broad-spectrum antibiotics either from the day of EAU induction or from one week prior to induction of disease.
The severity of the inflammation was assessed by fundus biomicroscopy or by histology, including immunohistology.
The immunophenotyping of T cells in local and distant lymph nodes was performed by flow cytometry.
We found that GF mice and mice where the microbiota was reduced one week before EAU induction were protected from severe autoimmune inflammation.
GF mice had lower numbers of infiltrating macrophages and significantly less T cell infiltration in the retina than CV mice with EAU.
GF mice also had reduced numbers of IFN-γ and IL-17-producing T cells and increased numbers of regulatory T cells in the eye-draining lymph nodes.
These data suggest that the presence of microbiota during autoantigen recognition regulates the inflammatory response by influencing the adaptive immune response.
American Psychological Association (APA)
Heissigerova, Jarmila& Seidler Stangova, Petra& Klimova, Aneta& Svozilkova, Petra& Hrncir, Tomas& Stepankova, Renata…[et al.]. 2016. The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108807
Modern Language Association (MLA)
Heissigerova, Jarmila…[et al.]. The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis. Journal of Immunology Research No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1108807
American Medical Association (AMA)
Heissigerova, Jarmila& Seidler Stangova, Petra& Klimova, Aneta& Svozilkova, Petra& Hrncir, Tomas& Stepankova, Renata…[et al.]. The Microbiota Determines Susceptibility to Experimental Autoimmune Uveoretinitis. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108807
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108807