Evaluation of Multiple Immunoassay Technology Platforms to Select the Anti-Drug Antibody Assay Exhibiting the Most Appropriate Drug and Target Tolerance

Joint Authors

Collet-Brose, Justine
Couble, Pierre-Jean
Deehan, Maureen R.
Nelson, Robert J.
Ferlin, Walter G.
Lory, Sabrina

Source

Journal of Immunology Research

Issue

Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2016-05-03

Country of Publication

Egypt

No. of Pages

15

Main Subjects

Biology

Abstract EN

The aim of this study was, at the assay development stage and thus with an appropriate degree of rigor, to select the most appropriate technology platform and sample pretreatment procedure for a clinical ADA assay.

Thus, ELISA, MSD, Gyrolab, and AlphaLISA immunoassay platforms were evaluated in association with target depletion and acid dissociation sample pretreatment steps.

An acid dissociation step successfully improved the drug tolerance for all 4 technology platforms and the required drug tolerance was achieved with the Gyrolab and MSD platforms.

The target tolerance was shown to be better for the ELISA format, where an acid dissociation treatment step alone was sufficient to achieve the desired target tolerance.

However, inclusion of a target depletion step in conjunction with the acid treatment raised the target tolerance to the desired level for all of the technologies.

A higher sensitivity was observed for the MSD and Gyrolab assays and the ELISA, MSD, and Gyrolab all displayed acceptable interdonor variability.

This study highlights the usefulness of evaluating the performance of different assay platforms at an early stage in the assay development process to aid in the selection of the best fit-for-purpose technology platform and sample pretreatment steps.

American Psychological Association (APA)

Collet-Brose, Justine& Couble, Pierre-Jean& Deehan, Maureen R.& Nelson, Robert J.& Ferlin, Walter G.& Lory, Sabrina. 2016. Evaluation of Multiple Immunoassay Technology Platforms to Select the Anti-Drug Antibody Assay Exhibiting the Most Appropriate Drug and Target Tolerance. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1108808

Modern Language Association (MLA)

Collet-Brose, Justine…[et al.]. Evaluation of Multiple Immunoassay Technology Platforms to Select the Anti-Drug Antibody Assay Exhibiting the Most Appropriate Drug and Target Tolerance. Journal of Immunology Research No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1108808

American Medical Association (AMA)

Collet-Brose, Justine& Couble, Pierre-Jean& Deehan, Maureen R.& Nelson, Robert J.& Ferlin, Walter G.& Lory, Sabrina. Evaluation of Multiple Immunoassay Technology Platforms to Select the Anti-Drug Antibody Assay Exhibiting the Most Appropriate Drug and Target Tolerance. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1108808

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1108808