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Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines
Joint Authors
Sprangers, Ben
Van Belle, Kristien
Herman, Jean
Waer, Mark
Louat, Thierry
Boon, Louis
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
B cell specific immunomodulatory drugs still remain an unmet medical need.
Utilisation of validated simplified in vitro models would allow readily obtaining new insights in the complexity of B cell regulation.
For this purpose we investigated which human B lymphocyte stimulation assays may be ideally suited to investigate new B lymphocyte immunosuppressants.
Primary polyclonal human B cells underwent in vitro stimulation and their proliferation, production of immunoglobulins (Igs) and of cytokines, and expression of cell surface molecules were analysed using various stimuli.
ODN2006, a toll-like receptor 9 (TLR9) agonist, was the most potent general B cell stimulus.
Subsequently, we investigated on which human B cell lines ODN2006 evoked the broadest immunostimulatory effects.
The Namalwa cell line proved to be the most responsive upon TLR9 stimulation and hence may serve as a relevant, homogeneous, and stable B cell model in an in vitro phenotypic assay for the discovery of new targets and inhibitors of the B cell activation processes.
As for the read-out for such screening assay, it is proposed that the expression of activation and costimulatory surface markers reliably reflects B lymphocyte activation.
American Psychological Association (APA)
Van Belle, Kristien& Herman, Jean& Boon, Louis& Waer, Mark& Sprangers, Ben& Louat, Thierry. 2016. Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1108815
Modern Language Association (MLA)
Van Belle, Kristien…[et al.]. Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines. Journal of Immunology Research No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1108815
American Medical Association (AMA)
Van Belle, Kristien& Herman, Jean& Boon, Louis& Waer, Mark& Sprangers, Ben& Louat, Thierry. Comparative In Vitro Immune Stimulation Analysis of Primary Human B Cells and B Cell Lines. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1108815
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108815