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Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation
Joint Authors
Mattsson, J.
Uhlin, M.
Sairafi, Darius
Stikvoort, Arwen
Gertow, Jens
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Graft-versus-host disease (GVHD) is a serious complication after allogeneic hematopoietic stem cell transplantation (HSCT).
We designed a functional assay for assessment of individual risk for acute GVHD.
Study Design and Methods.
Blood samples were collected from patients and donors before HSCT.
Two groups of seven patients each were selected, one in which individuals developed acute GVHD grades II–IV and one in which none showed any clinical signs of GVHD.
Peripheral blood mononuclear cells (PBMCs) isolated from donors were incubated in mixed lymphocyte cultures (MLCs) with recipient PBMCs.
The cells were characterized by flow cytometry before and after MLC.
Results.
Samples from donors in the GVHD group contained significantly lower frequencies of naïve γδ T-cells and T-cells expressing NK-cell markers CD56 and CD94.
Donor samples in this group also exhibited lower frequencies of naïve CD95+ T-cells compared to controls.
After MLC, there were dissimilarities in the CD4/CD8 T-cell ratio and frequency of CD69+ T-cells between the two patient groups, with the non-GVHD group showing higher frequencies of CD8+ and CD69+ T-cells.
Conclusion.
We conclude that a thorough flow cytometric analysis of donor cells for phenotype and allogeneic reactivity may be of value when assessing pretransplant risk for severe acute GVHD.
American Psychological Association (APA)
Sairafi, Darius& Stikvoort, Arwen& Gertow, Jens& Mattsson, J.& Uhlin, M.. 2016. Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108826
Modern Language Association (MLA)
Sairafi, Darius…[et al.]. Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation. Journal of Immunology Research No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1108826
American Medical Association (AMA)
Sairafi, Darius& Stikvoort, Arwen& Gertow, Jens& Mattsson, J.& Uhlin, M.. Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1108826
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108826